• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and experimental allergy : >Primary role of growth-related oncogene-alpha and granulocyte chemotactic protein-2 as neutrophil chemoattractants in chronic rhinosinusitis.
【24h】

Primary role of growth-related oncogene-alpha and granulocyte chemotactic protein-2 as neutrophil chemoattractants in chronic rhinosinusitis.

机译：在慢性鼻-鼻窦炎中，与生长相关的癌基因-α和粒细胞趋化蛋白2作为中性粒细胞趋化因子的主要作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The aetiology of chronic rhinosinusitis (CRS) remains unclear. The purpose of this study was to investigate neutrophil-attracting chemokine patterns in CRS without nasal polyposis. Methods The biological activity of the chemokines was identified using a two-step high-performance liquid chromatography (HPLC) technique combined with a bioassay in extracts from 55 CRS patients, and in the turbinate mucosa (TM) of patients (N=51) undergoing septumplasty. The biologic activity of each chemokine was assessed using blocking antibodies to chemokines. Immunolocalization of detected neutrophil chemokines was performed by quantitative evaluation of immunohistochemistry. Besides, PCR analysis was performed to quantify neutrophil chemokine mRNA. Results In CRS, the chemokines primarily detected by two-step HPLC were growth-related oncogene-alpha (GRO-alpha) and the granulocyte chemotactic protein-2 (GCP-2). Blocking of GCP-2 and GRO-alphad each resulted in chemotaxis inhibition rates of 43.3% and 35.9%, respectively, whereas anti-IL-8 and anti-ENA-78 had no effect. Both GCP-2 and GRO-alphad were generally synthesized by the surface epithelium and mucosal glands while GRO-alpha in particular was synthesized by endothelial cells, as shown by immunohistochemistry. The concentrations of the chemokines IL-8 and epithelial cell-derived neutrophil attractant-78 (ENA-78) were low in CRS and TM. Conclusion It appears that both GRO-alpha and GCP-2 contribute to neutrophil chemotaxis in CRS, whereas IL-8 and ENA-78 appear to be of secondary importance for the chemotaxis of neutrophils in this condition. The expression of chemokines in mucosal gland cells is the main phenomenon involved in constitutive neutrophil chemotaxis in the TM.
机译：背景慢性鼻鼻窦炎（CRS）的病因仍不清楚。这项研究的目的是调查没有鼻息肉的CRS中吸引中性粒细胞的趋化因子模式。方法采用两步高效液相色谱（HPLC）技术结合生物测定法，从55名CRS患者的提取物中以及鼻甲黏膜（TM）的患者（N = 51）中，对趋化因子的生物学活性进行了鉴定。隔膜成形术。使用针对趋化因子的封闭抗体评估每种趋化因子的生物学活性。通过免疫组织化学的定量评估进行检测到的中性粒细胞趋化因子的免疫定位。此外，进行PCR分析以定量中性粒细胞趋化因子mRNA。结果在CRS中，主要通过两步HPLC检测的趋化因子为生长相关癌基因α（GRO-alpha）和粒细胞趋化蛋白2（GCP-2）。分别阻断GCP-2和GRO-α导致趋化抑制率分别为43.3％和35.9％，而抗IL-8和抗ENA-78无效。如免疫组织化学所示，GCP-2和GRO-α一般都由表面上皮和粘膜腺合成，而GRO-α特别是由内皮细胞合成。在CRS和TM中，趋化因子IL-8和上皮细胞衍生的中性粒细胞引诱剂78（ENA-78）的浓度较低。结论在这种情况下，似乎GRO-α和GCP-2均对中性粒细胞的趋化性有贡献，而IL-8和ENA-78似乎对中性粒细胞的趋化性次要。趋化因子在粘膜腺细胞中的表达是TM中组成性中性粒细胞趋化性涉及的主要现象。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号