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首页> 外文期刊>Clinical and experimental allergy : >Sublingual immunotherapy reduces allergic symptoms in a mouse model of rhinitis.
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Sublingual immunotherapy reduces allergic symptoms in a mouse model of rhinitis.

机译：舌下免疫疗法可减轻鼻炎小鼠模型的过敏症状。

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BACKGROUND: Sublingual immunotherapy (SLIT) is a clinically effective treatment in both pollen and house dust mite-induced rhinitis and asthma. However, the mechanisms by which this is accomplished are not clear. OBJECTIVE: The objective of the current study was to establish a mouse model of rhinitis in order to study the effect and mechanisms of SLIT. METHODS: Mice were sensitized by intraperitoneal injections of alum-adsorbed Phleum pratense extract. Sensitized mice were SLIT-treated and subsequently challenged intranasally and analysed for clinical symptoms, antibody levels, eosinophilia and T cell response. RESULTS: Intranasal challenge of sensitized mice led to the development of rhinitis characterized by significantly increased sneezing and influx of eosinophils into the nose. Levels of specific IgE were fivefold increased in nasopharyngeal lavage (NAL) fluid and more than doubled in serum. Furthermore, a T-helper type 2 (Th2) like T cell response was observed in local draining lymph nodes. SLIT treatment of sensitized mice reduced sneezing, eosinophilia and IgE levels in the NAL by more than 50%. Moreover, serum levels of IgE and IgG1 as well as T cell response in the draining lymph nodes were also significantly reduced. Treatment for a shorter time or with a lower dose only led to minor reductions of the clinical and immunological parameters, indicating that the effect of SLIT is time and dose dependent. CONCLUSION: In the present study, we have established a mouse model displaying the hallmarks of allergic rhinitis using a clinically relevant allergen. Using this model, we have demonstrated that SLIT treatment is able to reduce allergic symptoms in a time- and dose-dependent manner.
机译：背景：舌下免疫疗法（SLIT）在花粉和屋尘螨诱发的鼻炎和哮喘中都是临床有效的治疗方法。但是，实现这一目标的机制尚不清楚。目的：本研究的目的是建立一种鼻炎小鼠模型，以研究SLIT的作用及其机制。方法：通过腹膜内注射铝吸附的Ph草提取物致敏小鼠。对致敏的小鼠进行SLIT处理，然后鼻内攻击，并分析其临床症状，抗体水平，嗜酸性粒细胞增多和T细胞反应。结果：致敏小鼠的鼻内刺激导致鼻炎的发展，其特征是打喷嚏和嗜酸性粒细胞大量流入鼻子。鼻咽灌洗液中的特异性IgE水平增加了五倍，血清中的特异性IgE水平增加了一倍以上。此外，在局部引流淋巴结中观察到类似T细胞反应的T型辅助2型（Th2）。对致敏小鼠的SLIT治疗可将NAL中的打喷嚏，嗜酸性粒细胞增多和IgE水平降低50％以上。此外，引流淋巴结中的IgE和IgG1血清水平以及T细胞反应也显着降低。较短时间或较低剂量的治疗只会导致临床和免疫学参数的轻微降低，这表明SLIT的作用取决于时间和剂量。结论：在本研究中，我们建立了一种小鼠模型，该模型使用临床相关的过敏原来显示过敏性鼻炎的标志。使用该模型，我们证明了SLIT治疗能够以时间和剂量依赖的方式减轻过敏症状。

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