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首页> 外文期刊>Clinical and experimental allergy : >The 2S albumin allergens of Arachis hypogaea, Ara h 2 and Ara h 6, are the major elicitors of anaphylaxis and can effectively desensitize peanut-allergic mice
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The 2S albumin allergens of Arachis hypogaea, Ara h 2 and Ara h 6, are the major elicitors of anaphylaxis and can effectively desensitize peanut-allergic mice

机译：花生的2S白蛋白变应原，Ara h 2和Ara h 6是引起过敏反应的主要诱因，可以有效地使花生过敏小鼠脱敏

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Background: Ara h 2 and Ara h 6, co-purified together in a 13-25 kD fraction (Ara h 2/6; 20 kD fraction) on gel filtration chromatography, account for the majority of effector activity in a crude peanut extract (CPE) when assayed with RBL SX-38 cells sensitized with IgE from human peanut allergic sera. Objectives: To determine if Ara h 2/6 are the primary peanut allergens responsible for allergic reactions in vivo and to determine if Ara h 2/6 would be sufficient to prevent allergic reactions to a complete CPE. Methods: An oral sensitization mouse model of peanut allergy was used to assess the activity of Ara h 2/6 (20 kD) and CPE without the 20 kD fraction (CPE w/o 20 kD) for allergic provocation challenge and immunotherapy. The activity of these preparations was also tested in an assay of histamine release from human basophils in whole blood. Results: Compared with mice challenged with control CPE, mice challenged with CPE w/o 20 kD experienced reduced symptoms (P < 0.05) and a smaller decrease in body temperature (P < 0.01). Results with the basophil histamine release assay corroborated these findings (P < 0.01). The mouse model was also used to administer Ara h 2/6 (20 kD) in an immunotherapy protocol, in which peanut-allergic mice treated with the 20 kD fraction experienced significantly reduced symptoms, changes in body temperature, and mast cell protease (MMCP-1) release compared with placebo (P < 0.01 for all parameters). Importantly, immunotherapy with the 20 kD fraction was just as effective as treatment with CPE, whereas CPE w/o 20 kD was significantly less effective for higher dose peanut challenges. Conclusions and Clinical Relevance: Ara h 2/6 are the most potent peanut allergens in vivo and can be used to desensitize peanut-allergic mice. These results have potential implications for clinical research in the areas of diagnosis and immunotherapy for peanut allergy.
机译：背景：Ara h 2和Ara h 6在凝胶过滤色谱上以13-25 kD的馏分（Ara h 2/6; 20 kD的馏分）共同纯化，占粗制花生提取物（ （CPE）时，用来自人花生过敏性血清的IgE致敏的RBL SX-38细胞进行分析。目的：确定Ara h 2/6是否是引起体内过敏反应的主要花生过敏原，并确定Ara h 2/6是否足以阻止对完全CPE的过敏反应。方法：使用花生过敏的口服敏化小鼠模型评估Ara h 2/6（20 kD）和不含20 kD分数（CPE w / o 20 kD）的CPE的活性，以进行激发性激发和免疫治疗。还通过测定全血中人类嗜碱性粒细胞释放组胺来测试这些制剂的活性。结果：与用对照CPE攻击的小鼠相比，用CPE w / o 20 kD攻击的小鼠的症状减轻（P <0.05），体温下降较小（P <0.01）。嗜碱性粒细胞组胺释放测定的结果证实了这些发现（P <0.01）。小鼠模型还用于免疫治疗方案中的Ara h 2/6（20 kD）给药，其中以20 kD分数处理的花生过敏小鼠的症状，体温变化和肥大细胞蛋白酶（MMCP）明显降低-1）与安慰剂相比释放（所有参数的P <0.01）。重要的是，使用20 kD分数的免疫疗法与CPE疗法一样有效，而CPE w / o 20 kD则对更高剂量的花生挑战明显无效。结论和临床意义：Ara h 2/6是体内最有效的花生过敏原，可用于使花生过敏小鼠脱敏。这些结果对花生过敏的诊断和免疫治疗领域的临床研究具有潜在的意义。

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