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首页> 外文期刊>Clinical and experimental allergy : >Quantification of circulating house dust mite-specific IL-4- and IL-13-secreting T cells correlates with rhinitis severity in asthmatic children and varies with the seasons
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Quantification of circulating house dust mite-specific IL-4- and IL-13-secreting T cells correlates with rhinitis severity in asthmatic children and varies with the seasons

机译：哮喘儿童中循环性屋尘螨特异性IL-4-和IL-13分泌性T细胞的定量与鼻炎严重程度相关，并随季节而变化

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Background: Defining suitable markers to diagnose and monitor allergy and its severity is essential to correctly assign patients for specific immunotherapy. Circulating levels of specific IgE are good markers of sensitization, but not of clinically symptomatic allergy. Objective: To quantify circulating interleukin (IL)-4- and IL-13-secreting T cells specific for house dust mite (HDM) in children presenting HDM-allergic asthma associated or not with rhinitis and correlate results with clinical symptoms. Methods: We analysed 26 children with HDM respiratory disease (allergic rhinitis and asthma) together with six children with non-allergic asthma. Peripheral blood mononuclear cells were stimulated with HDM extract in a 24-h ELISpot assay to quantify the number of HDM-specific IL-4- and IL-13-secreting T cells. Asthma severity and control, and rhinitis severity were scored according to the Global Initiative for Asthma (GINA) and the Allergic Rhinitis and its Impact on Asthma (ARIA) Guidelines. Results: The number of HDM-specific IL-4- and IL-13-secreting T cells was higher in patients with allergic asthma as compared to patients with non-allergic asthma. It varied with the season of blood sampling with two peaks in the fall and early spring. Independently of the season, the number of HDM-specific IL-4-secreting T cells correlated with rhinitis severity (OR = 2; 95% IC:1.1-3.8; P = 0.04). Conclusions and Clinical relevance: Allergen-specific IL-4- and IL-13-producing T cells were only detected in HDM-allergic asthmatic children (not in patients with non-allergic asthma). Their numbers correlated with clinical severity of allergic rhinitis.
机译：背景：定义合适的标记物以诊断和监测过敏及其严重程度对于正确分配患者进行特异性免疫治疗至关重要。循环中的特定IgE水平是敏化的良好标志，但不是临床症状过敏的良好标志。目的：对患有HDM过敏性哮喘（伴发或不伴有鼻炎）的儿童量化屋尘螨（HDM）特异性分泌循环白细胞介素（IL）-4-和分泌IL-13的T细胞，并将结果与​​临床症状相关联。方法：我们分析了26例HDM呼吸系统疾病（过敏性鼻炎和哮喘）以及6例非过敏性哮喘儿童。在24小时ELISpot分析中，用HDM提取物刺激外周血单核细胞，以量化HDM特异性分泌IL-4和IL-13的T细胞的数量。根据全球哮喘倡议（GINA）和过敏性鼻炎及其对哮喘的影响（ARIA）指南对哮喘的严重程度和控制以及鼻炎的严重程度进行评分。结果：与非过敏性哮喘患者相比，过敏性哮喘患者HDM特异性分泌IL-4和IL-13的T细胞数量更高。它随采血季节而变化，在秋季和早春有两个峰值。与季节无关，HDM特异性分泌IL-4的T细胞数量与鼻炎严重程度相关（OR = 2; 95％IC：1.1-3.8; P = 0.04）。结论和临床意义：仅在HDM过敏性哮喘儿童（非过敏性哮喘患者中）中检测到产生过敏原的IL-4-和IL-13产生的T细胞。它们的数量与变应性鼻炎的临床严重程度相关。

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