• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and experimental allergy : >Nasal mucosal expression of the leukotriene and prostanoid pathways in seasonal and perennial allergic rhinitis.
【24h】

Nasal mucosal expression of the leukotriene and prostanoid pathways in seasonal and perennial allergic rhinitis.

机译：季节性和多年生变应性鼻炎中白三烯和前列腺素途径的鼻黏膜表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Leukotrienes (LTs) and prostanoids are potent pro-inflammatory and vasoactive lipid mediators implicated in airway disease, but their cellular sources in the nasal airway in naturally occurring allergic rhinitis (AR) are unclear. OBJECTIVE: To quantify cellular expression of enzymes of the 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways by immunohistochemistry in nasal biopsies from patients with symptomatic perennial AR (PAR, n = 13) and seasonal AR (SAR, n = 14) and from normal subjects (n = 12). METHODS: Enzymes of the 5-LO pathway (5-LO, FLAP, LT A4 hydrolase, LTC4 synthase) and the COX pathway (COX-1, COX-2, prostaglandin D2 synthase) were immunostained in glycol methacrylate resin-embedded inferior turbinate biopsy specimens, quantified in the lamina propria and epithelium, and co-localized to leucocyte markers by camera lucida. RESULTS: In the lamina propria of PAR biopsies, median counts of cells expressing FLAP were fourfold higher than in normal biopsies (Mann-Whitney, P = 0.014), and also tended to be higher than in SAR biopsies (P = 0.06), which were not different from normal. PAR biopsies showed threefold more cells immunostaining for LTC4 synthase compared with SAR biopsies (P = 0.011) but this was not significant compared with normal biopsies (P = 0.2). These changes were associated with ninefold more eosinophils (P = 0.0005) with no differences in other leucocytes. There were no significant differences in the lamina propria in immunostaining for 5-LO, LTA4 hydrolase, COX-1, COX-2 or PGD2 synthase. Within the epithelium, increased expression of COX-1 was evident in PAR biopsies (P = 0.014) and SAR biopsies (P = 0.037), associated with more intra-epithelial mast cells in both rhinitic groups (P < 0.02). CONCLUSIONS: In the nasal biopsies of PAR subjects, increased expression of regulatory enzymes of the cysteinyl-LT biosynthetic pathway was associated with lamina propria infiltration by eosinophils. Seasonal rhinitis biopsies shared only some of these changes, consistent with transient disease. Increased intra-epithelial mast cells and epithelial COX-1 expression in both rhinitic groups may generate modulatory prostanoids.
机译：背景：白三烯（LTs）和类前列腺素是强力的促炎和血管活性脂质介质，与气道疾病有关，但在自然发生的变应性鼻炎（AR）中，它们在鼻道中的细胞来源尚不清楚。目的：通过免疫组织化学定量分析有症状常年性AR（PAR，n = 13）和季节性AR（SAR，n =）患者鼻活检中5-脂氧合酶（5-LO）和环氧合酶（COX）途径的酶的细胞表达14）和正常受试者（n = 12）。方法：将5-LO途径的酶（5-LO，FLAP，LT A4水解酶，LTC4合酶）和COX途径的酶（COX-1，COX-2，前列腺素D2合酶）用甲基丙烯酸乙二醇酯树脂包埋的下鼻甲进行免疫染色。活检标本，在固有层和上皮中定量，并通过照相机lucida共定位于白细胞标记。结果：在PAR活检的固有层中，表达FLAP的细胞的中位数比正常活检高出四倍（Mann-Whitney，P = 0.014），并且也往往比SAR活检高（P = 0.06）。与正常情况没有什么不同。 PAR活检显示，与SAR活检相比，对LTC4合酶的细胞免疫染色增加了三倍（P = 0.011），但与正常活检相比（P = 0.2）并不显着。这些变化与嗜酸性粒细胞增加了九倍（P = 0.0005），其他白细胞没有差异。在5-LO，LTA4水解酶，COX-1，COX-2或PGD2合酶的免疫染色中，固有层没有显着差异。在上皮内，PAR活检（P = 0.014）和SAR活检（P = 0.037）中COX-1的表达明显增加，这与两个鼻炎组的上皮内肥大细胞相关（P <0.02）。结论：在PAR受试者的鼻活检中，半胱氨酸-LT生物合成途径的调节酶表达增加与嗜酸性粒细胞浸润固有层有关。季节性鼻炎活检仅分享其中一些变化，与短暂性疾病一致。鼻基组中上皮内肥大细胞增加和上皮COX-1表达可能产生调节性前列腺素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号