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首页> 外文期刊>Цитология >Effect of narrow fractions of chromatin non-histone proteins on the expression of membrane tumor-associated antigen MA-50 and phosphorylation of proteins of cultivated rat hepatocytes
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Effect of narrow fractions of chromatin non-histone proteins on the expression of membrane tumor-associated antigen MA-50 and phosphorylation of proteins of cultivated rat hepatocytes

机译：染色质非组蛋白窄片段对膜肿瘤相关抗原MA-50表达和培养大鼠肝细胞蛋白质磷酸化的影响

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摘要

As earlier reported, the main component of narrow fractions of chromosomal non-histone proteins (NHP) of kidney and of Zaidel hepatoma cells has its own protein kinase activity, and is identified as a heteroorgan NHP-antigen, which is intrinsic to the definite renal tissue and absent in the liver. Effects of narrow fractions of kidney and Zaidel hepatoma NHP on biosynthetic processes and sizes of hepatocytes were studied in vitro. It has been shown that as a result of a 5h incubation of rat hepatocytes with a narrow fraction of renal NHP the proportion of small hepatocytes increases approximately by 12 percent as compared with that of cells cultivated without NHP. Besides, binding of organ-specific anti-kidney immune serum with a small hepatocytepopulation rises by more than 20 percent, which results from the expression of tumor-associated heteroorgan kidney-specific antigen on the hepatocyte surface. According to immunoprecipitation and subsequent electrophoresis, the molecular mass of a membrane heteroorgan antigen on the surface of hepatocytes amounts approximately to 65 kDa, and an active phosphorylation of cellular proteins takes place. The same effect on hepatocytes is produced by a narrow NHP fraction of chromatin of Zaidel hepatoma cells, whereas no phosphorylation is observed in the presence of liver NHP as well as in the absence of NHP. It is suggested that the heteroorgan NHP-antigen induces biosynthetic processes including synthesis of membrane tumorassociated antigen on the sufaceof hepatocytes cultivated in vitro by activation of cellular protein phosphorylation, which can lead to changes in size of cultivated cells.
机译：如较早的报道，肾脏和Zaidel肝癌细胞的染色体非组蛋白蛋白（NHP）的狭窄部分的主要成分具有其自身的蛋白激酶活性，并被确定为异器官NHP抗原，这是确定性肾脏固有的组织和肝脏中不存在。体外研究了肾脏和Zaidel肝癌NHP的狭窄部分对生物合成过程和肝细胞大小的影响。已经显示，将大鼠肝细胞与窄比例的肾NHP孵育5h，与不培养NHP的细胞相比，小肝细胞的比例增加了约12％。此外，器官特异性抗肾脏免疫血清与少量肝细胞的结合增加了20％以上，这是由于肿瘤相关的异器官肾脏特异性抗原在肝细胞表面的表达所致。根据免疫沉淀和随后的电泳，肝细胞表面上的膜异器官抗原的分子量约为65 kDa，并且发生了细胞蛋白的活性磷酸化。 Zaidel肝细胞瘤细胞的染色质的NHP比例很小，对肝细胞产生相同的作用，而在有肝NHP和没有NHP的情况下均未观察到磷酸化。提示异源NHP抗原通过激活细胞蛋白磷酸化而诱导生物合成过程，包括在体外培养的肝细胞表面合成膜肿瘤相关抗原，这可能导致培养细胞大小的改变。

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