...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Increased activation of protein kinase C with cubic phase lipid compared with liposomes
【24h】

Increased activation of protein kinase C with cubic phase lipid compared with liposomes

机译：与脂质体相比，具有立方相脂质的蛋白激酶C的激活增加

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein kinase C (PKC) activation is measured using Liposomes containing phosphatidylserine. Certain lipids display a wide range of polymorphism, depending on conditions. They can give rise to nonlamellar phases, such as hexagonal or cubic phases, as well as to lamellar phases. In this paper, we studied the activity and membrane binding of PKC in lipid bicontinuous cubic phases and hexagonal phases. The cubic phase Lipid systems were (1) monoolein with 1-palmitoyl-2-oleoyl-3-phosphatidylserine (MO/PS) and (2) dielaidoylphosphatidylethanolamine/alamethicin (DEPE/alamethicin). Under fully hydrated conditions, both of the above Lipid mixtures are bicontinuous cubic phases with a space group of Pn3m within certain concentration ratios and temperature ranges. Dioleoylphosphatidylethanolamine (DOPE) with up to 10 mol % PS exists in the hexagonal phase at room temperature. These cubic and hexagonal phases were able to support the PKC-catalyzed phosphorylation of histone. The amount of PKC bound to the MO/PS cubic phase showed Little increase between 5 and 10 mol % PS. For both of the cubic phase systems studied, only a minor fraction of the PKC was bound to the membrane. This indicates that the specific activity of the enzyme bound to cubic phase membranes is much greater than that bound to phospholipid in the lamellar phase, Addition of up to 50 mol % MO to lipid in the lamellar phase had relatively small effects on the activity of PKC. The increase in PKC activity correlated well with an increase in PKC binding, resulting in little change in the specific activity of the membrane-bound form. These findings may be physiologically relevant due to the apparent presence of the cubic phase in certain biological structures. Also, these phases have little or no curvature strain, a property which has been shown to correlate with activation of PKC. Therefore, other factors, such as a curved morphology and/or interfacial polarity, must be responsible for the activation of PKC in these Lipid systems. [References: 47]
机译：使用含有磷脂酰丝氨酸的脂质体来测量蛋白激酶C（PKC）的活化。某些脂质根据条件表现出广泛的多态性。它们可以产生非层状相，例如六方相或立方相，以及层状相。在本文中，我们研究了脂质双连续立方相和六方相中PKC的活性和膜结合。立方相脂质系统是（1）具有1-棕榈酰基-2-油酰基-3-磷脂酰丝氨酸（MO / PS）的单油精和（2）二烯丙基磷脂酰乙醇胺/阿洛米辛（DEPE /阿拉米辛）。在完全水合的条件下，上述两种脂质混合物都是双连续立方相，在一定的浓度比和温度范围内，其空间群为Pn3m。在室温下，六方相中存在PS含量最高为10 mol％的二油酰基磷脂酰乙醇胺（DOPE）。这些立方相和六方相能够支持PKC催化的组蛋白磷酸化。与MO / PS立方相结合的PKC量在5至10 mol％PS之间几乎没有增加。对于所研究的两个立方相系统，只有一小部分PKC与膜结合。这表明与立方相膜结合的酶的比活性比在层状相中结合的磷脂的比活性大得多。在层状相中的脂质中添加高达50 mol％的MO对PKC的活性影响相对较小。 。 PKC活性的增加与PKC结合的增加紧密相关，导致膜结合形式的比活性几乎没有变化。由于某些生物结构中明显存在立方相，因此这些发现可能与生理相关。而且，这些相几乎没有曲率应变，或者没有曲率应变，已经证明该性质与PKC的活化相关。因此，其他因素，例如弯曲的形态和/或界面极性，必须负责这些脂质系统中PKC的活化。 [参考：47]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号