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首页> 外文期刊>Biochemistry >Peptide-membrane interactions studied by a new phospholipid/polydiacetylene colorimetric vesicle assay.
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Peptide-membrane interactions studied by a new phospholipid/polydiacetylene colorimetric vesicle assay.

机译：通过新的磷脂/聚二乙炔比色囊泡试验研究了肽-膜相互作用。

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摘要

Interactions between peptides and lipid membranes play major roles in numerous physiological processes, such as signaling, cytolysis, formation of ion channels, and cellular recognition. We describe a new colorimetric technique for studying peptide-membrane interactions. The new assay is based on supramolecular assemblies composed of phospholipids embedded in a matrix of polydiacetylene (PDA) molecules. The phospholipid/PDA vesicle solutions undergo visible color changes upon binding of membrane peptides. Experiments utilizing various analytical techniques confirm that the blue-to-red color transitions of the phospholipid/PDA vesicles are directly related to adoption of helical conformations by the peptides and their association with the lipids. Spectroscopic data indicate that the colorimetric transitions are correlated with important molecular parameters, such as the degree of penetration of the peptides into lipid bilayers, and the mechanisms of peptide-lipid binding. The results suggest that the new colorimetric assay could be utilized for studying interactions and organization of membrane peptides.
机译：肽与脂质膜之间的相互作用在许多生理过程中起着重要作用，例如信号传导，细胞溶解，离子通道的形成和细胞识别。我们描述了一种新的比色技术，用于研究肽膜相互作用。新的测定基于超分​​子组装体，该组装体由嵌入聚二乙炔（PDA）分子基质中的磷脂组成。磷脂/ PDA囊泡溶液在结合膜肽后会发生可见的颜色变化。利用各种分析技术进行的实验证实，磷脂/ PDA囊泡的蓝色至红色过渡与肽对螺旋构象的采用及其与脂质的缔合直接相关。光谱数据表明，比色转变与重要的分子参数相关，例如肽渗透到脂质双层中的程度以及肽-脂质结合的机理。结果表明，该新的比色测定法可用于研究膜肽的相互作用和组织。

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