Identification f Asp218 and Asp326 as the Principal Mg~(2+) Binding Ligands of the Homing Endonuclease PI-SceI

Sylvia Schottler; Wolfgang Wende; Vera Pingoud

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Identification f Asp218 and Asp326 as the Principal Mg~(2+) Binding Ligands of the Homing Endonuclease PI-SceI

机译：鉴定f Asp218和Asp326为归巢核酸内切酶PI-SceI的主要Mg〜（2+）结合配体

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The monomeric homing endonuclease PI-SceI harbors two catalytic centers which cooperate in the cleavae of the two strands of its extended recognition sequence.

机译：单体归巢核酸内切酶PI-SceI包含两个催化中心，它们在其扩展识别序列的两条链的切割中协同作用。

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《Biochemistry》 |2000年第51期|共6页
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Sylvia Schottler; Wolfgang Wende; Vera Pingoud;
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1. 樟巢螟雌蛾性信息素主要组分鉴定及诱蛾活性 [J] . 何月秋, 谢红丽, 池树友农业科学与技术（英文版） . 2016,第004期
2. Identification f Asp218 and Asp326 as the Principal Mg~(2+) Binding Ligands of the Homing Endonuclease PI-SceI [J] . Sylvia Schottler, Wolfgang Wende, Vera Pingoud Biochemistry . 2000,第51期

机译：鉴定f Asp218和Asp326为归巢核酸内切酶PI-SceI的主要Mg〜（2+）结合配体
3. Chimeras of the Homing Endonuclease PI-SceI and the Homologous Candida tropicalis Intein: A Study to Explore the Possibility of Exchanging DNA-Binding Modules to Obtain Highly Specific Endonucleases with Altered Specificity [J] . Shawn Steuer, Vera Pingould, Alfred Pingoud, Chembiochem: A European journal of chemical biology . 2004,第2期

机译：归巢核酸内切酶PI-SceI和热带假丝酵母Intein的嵌合体：研究交换DNA绑定模块获得具有改变的特异性的高度特异性核酸内切酶的可能性的研究。
4. Mapping of a DNA binding region of the PI-sceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis. [J] . Hu D, Crist M, Duan X, Biochemistry . 1999,第39期

机译：通过亲和力裂解和丙氨酸扫描诱变对PI-sceI归巢核酸内切酶的DNA结合区域进行定位。
5. Identification of ligand-receptor binding affinity using AlGaN/GaN high electron mobility transistors and binding-site models [C] . Wang Yu-Lin, Chih-Cheng Huang, You-Ren Hsu, IEEE International Conference on Nano/Micro Engineered and Molecular Systems . 2013

机译：使用AlGaN / GaN高电子迁移率晶体管和结合位点模型鉴定配体-受体结合亲和力
6. Identification of protein-ligand binding sites by top-down mass spectrometry. [D] . Yin, Sheng. 2010

机译：通过自上而下的质谱鉴定蛋白质-配体结合位点。
7. The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. [O] . W Grindl, W Wende, V Pingoud, 1998

机译：归巢核酸内切酶PI-sceI的蛋白质剪接域负责特异性DNA结合。
8. The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. [O] . Grindl, W, Wende, W, Pingoud, V, 1998

机译：归巢核酸内切酶PI-sceI的蛋白质剪接域负责特异性DNA结合。

Identification f Asp218 and Asp326 as the Principal Mg~(2+) Binding Ligands of the Homing Endonuclease PI-SceI

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