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首页> 外文期刊>Biochemistry >Use of a photoactivated ruthenium dimer complex to measure electron transfer between the Rieske iron-sulfur protein and cytochrome c(1) in the cytochrome bc(1) complex.
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Use of a photoactivated ruthenium dimer complex to measure electron transfer between the Rieske iron-sulfur protein and cytochrome c(1) in the cytochrome bc(1) complex.

机译：使用光活化的钌二聚体复合物来测量Rieske铁硫蛋白与细胞色素bc（1）复合物中的细胞色素c（1）之间的电子转移。

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摘要

Electron transfer between the Rieske iron-sulfur protein (Fe(2)S(2)) and cytochrome c(1) was studied using the ruthenium dimer, Ru(2)D, to either photoreduce or photooxidize cytochrome c(1) within 1 micros. Ru(2)D has a charge of +4, which allows it to bind with high affinity to the cytochrome bc(1) complex. Flash photolysis of a solution containing beef cytochrome bc(1), Ru(2)D, and a sacrificial donor resulted in reduction of cytochrome c(1) within 1 micros, followed by electron transfer from cytochrome c(1) to Fe(2)S(2) with a rate constant of 90,000 s(-1). Flash photolysis of reduced beef bc(1), Ru(2)D, and a sacrificial acceptor resulted in oxidation of cytochrome c(1) within 1 micros, followed by electron transfer from Fe(2)S(2) to cytochrome c(1) with a rate constant of 16,000 s(-1). Oxidant-induced reduction of cytochrome b(H) was observed with a rate constant of 250 s(-1) in the presence of antimycin A. Electron transfer from Fe(2)S(2) to cytochrome c(1) within the Rhodobacter sphaeroides cyt bc(1) complex was found to have a rate constant of 60,000 s(-1) at 25 degrees C, while reduction of cytochrome b(H) occurred with a rate constant of 1000 s(-1). Double mutation of Ala-46 and Ala-48 in the neck region of the Rieske protein to prolines resulted in a decrease in the rate constants for both cyt c(1) and cyt b(H) reduction to 25 s(-1), indicating that a conformational change in the Rieske protein has become rate-limiting.
机译：使用钌二聚体Ru（2）D研究了Rieske铁硫蛋白（Fe（2）S（2））和细胞色素c（1）之间的电子转移，以光还原或光氧化1内的细胞色素c（1）微米。 Ru（2）D的电荷为+4，可使其以高亲和力与细胞色素bc（1）复合物结合。含有牛肉细胞色素bc（1），Ru（2）D和牺牲性供体的溶液的快速光解导致细胞色素c（1）在1微米内减少，然后电子从细胞色素c（1）转移到Fe（2） S（2），速率常数为90,000 s（-1）。还原牛肉bc（1），Ru（2）D和牺牲性受体的快速光解导致细胞色素c（1）在1微米内氧化，然后电子从Fe（2）S（2）转移到细胞色素c（ 1）的速率常数为16,000 s（-1）。在存在抗霉素A的情况下，观察到氧化剂诱导的细胞色素b（H）还原，速率常数为250 s（-1）。在红细菌中电子从Fe（2）S（2）转移到细胞色素c（1）发现sphaeroides cyt bc（1）复合物在25摄氏度时的速率常数为60,000 s（-1），而细胞色素b（H）的还原速率为1000 s（-1）。 Rieske蛋白颈部区域的Ala-46和Ala-48双重突变为脯氨酸，导致cyt c（1）和cyt b（H）还原至25 s（-1）的速率常数降低，表明Rieske蛋白的构象变化已成为限速。

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