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首页> 外文期刊>Biochemistry >Suppression of microtubule dynamic instability and treadmilling by deuterium oxide.
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Suppression of microtubule dynamic instability and treadmilling by deuterium oxide.

机译：氧化氘抑制微管动态不稳定性和跑步。

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摘要

Deuterium oxide (D(2)O) is known to promote the assembly of tubulin into microtubules in vitro, to increase the volume of mitotic spindles and the number and length of spindle microtubules, and to inhibit mitosis. Reasoning that its actions on cellular microtubules could be due to modulation of microtubule dynamics, we examined the effects of replacing H(2)O with D(2)O on microtubule dynamic instability, treadmilling, and steady-state GTPase activity. We found that replacing 50% or more of the H(2)O with D(2)O promoted microtubule polymerization and stabilized microtubules against dilution-induced disassembly. Using steady-state axoneme-seeded microtubules composed of pure tubulin and video microscopy, we found that 84% D(2)O decreased the catastrophe frequency by 89%, the shortening rate by 80%, the growing rate by 50%, and the dynamicity by 93%. Sixty percent D(2)O decreased the treadmilling rate of microtubules composed of tubulin and microtubule-associated proteins by 42%, and 89% D(2)O decreased the steady-state GTP hydrolysis rate by 90%. The mechanism responsible for the ability of D(2)O to stabilize microtubule dynamics may involve enhancement of hydrophobic interactions in the microtubule lattice and/or the substitution of deuterium bonds for hydrogen bonds.
机译：已知氧化氘（D（2）O）促进微管蛋白在体外组装成微管，增加有丝分裂纺锤体的体积以及纺锤体微管的数量和长度，并抑制有丝分裂。由于其对细胞微管的作用可能是由于微管动力学的调节，因此我们检查了用D（2）O代替H（2）O对微管动态不稳定性，跑步机和稳态GTPase活性的影响。我们发现，用D（2）O代替50％或更多的H（2）O可以促进微管聚合并稳定微管，防止稀释引起的拆卸。使用由纯微管蛋白和视频显微镜组成的稳态轴突接种微管，我们发现84％的D（2）O使突变频率降低了89％，缩短率降低了80％，生长率降低了50％，并且动态性提高了93％。 60％的D（2）O将微管蛋白和微管相关蛋白组成的微管的跑步速度降低了42％，而89％的D（2）O将稳态GTP水解速度降低了90％。负责D（2）O稳定微管动力学能力的机制可能涉及增强微管晶格中的疏水相互作用和/或氘键取代氢键。

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