Identification of Lipid-Accessible Sites on the Nephrops 16-kDa Proteolipid Incorporated into a Hybrid Vacuolar H~+-ATPase: Site-Directed Labeling with N-(1-Pyrenyl)cyclohexylcarbodiimide and Fluorescence Quenching Analysis

Michael Harrison; Ben Powell; Macolm E. Finbow

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Identification of Lipid-Accessible Sites on the Nephrops 16-kDa Proteolipid Incorporated into a Hybrid Vacuolar H~+-ATPase: Site-Directed Labeling with N-(1-Pyrenyl)cyclohexylcarbodiimide and Fluorescence Quenching Analysis

机译：Nephroplar H〜+ -ATPase中掺入的Nephrops 16-kDa蛋白脂质上脂类可及位点的鉴定：N-（1-吡啶基）环己基碳二亚胺的定点标记和荧光猝灭分析

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Proton translocation by the vacuolar H~+-ATPase is mediated by a multicopy transmembrane protein, the 16-kDa proteolipid.

机译：液泡H + ATP酶的质子易位是由多拷贝跨膜蛋白16 kDa蛋白脂介导的。

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《Biochemistry》 |2000年第35期|共7页
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Michael Harrison; Ben Powell; Macolm E. Finbow;
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1. Identification of lipid-accessible sites on the nephrops 16-kDa proteolipid incorporated into a hybrid vacuolar H(+)-ATPase: site-directed labeling with N-(1-Pyrenyl)cyclohexylcarbodiimide and fluorescence quenching analysis. [J] . Harrison M, Powell B, Finbow ME, Biochemistry . 2000,第25期

机译：掺入杂化液泡H（+）-ATPase的肾小球16-kDa蛋白脂质上脂质可及位点的鉴定：用N-（1-1-基）环己基碳二亚胺定点标记和荧光猝灭分析。
2. Identification of Lipid-Accessible Sites on the Nephrops 16-kDa Proteolipid Incorporated into a Hybrid Vacuolar H~+-ATPase: Site-Directed Labeling with N-(1-Pyrenyl)cyclohexylcarbodiimide and Fluorescence Quenching Analysis [J] . Michael Harrison, Ben Powell, Macolm E. Finbow Biochemistry . 2000,第35期

机译：Nephroplar H〜+ -ATPase中掺入的Nephrops 16-kDa蛋白脂质上脂类可及位点的鉴定：N-（1-吡啶基）环己基碳二亚胺的定点标记和荧光猝灭分析
3. High-Throughput Protein Structural Analysis Using Site-Directed Fluorescence Labeling and the Bimane Derivative (2-Pyridyl)dithiobimane. [J] . Mansoor SE, Farrens DL Biochemistry . 2004,第29期

机译：使用定点荧光标记和Bimane衍生物（2-Pyridyl）dithiobimane的高通量蛋白质结构分析。

Identification of Lipid-Accessible Sites on the Nephrops 16-kDa Proteolipid Incorporated into a Hybrid Vacuolar H~+-ATPase: Site-Directed Labeling with N-(1-Pyrenyl)cyclohexylcarbodiimide and Fluorescence Quenching Analysis

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