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首页> 外文期刊>Biochemistry >Correlation between Processing Efficiency for Ribonuclease P Minimal Substrates and Conformation of the Nucleotide-1 at the Cleavage Position
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Correlation between Processing Efficiency for Ribonuclease P Minimal Substrates and Conformation of the Nucleotide-1 at the Cleavage Position

机译：核糖核酸酶P最小底物的加工效率与切割位点处核苷酸1的构型之间的相关性

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摘要

It is demonstrated that acceptor stem duplexes derived from native tRNAs which contain athree-nucleotide extension at the Y-terminus of mature tRNA are minimal substrates for ribonuclease pfrom both ESCher-ichia coli and Bacillus subulb-Variants with a cyUdine at position一1are most efficientlyprocessed whereas the G -lvariant represents a comparatively poor substrate.An A-lacceptor stemvanantIs a slightly better substrate than the G-lvariant though generally distinctly less efficient thanthe C一lduplex.This is in qualitative agreement with the frequency of the occurrence of the correspondingnucleotides at position一lin naturalsubstrates,which is highest for pyrimidines and least for G.NMRanalyses of the cozesponding acceptor stems reveal that the confonEation of the nuckotides at position-lcorrelates with the substrate preferences of Ribonuclease P:Whereas C-1adopts a conformationcharacterized by a glycOSidic angle in the anti range (close to high-GMi),the G -1is clearly in synconformation,and that of A-lis intermediate between high-anti and syn-The riboses of nucleotides-lare in all cases predominantly 2'-endo puckered.
机译：结果表明，来自天然tRNA的受体茎双链体在成熟tRNA的Y端Y端含有三核苷酸延伸，是来自大肠杆菌和芽孢杆菌亚变种的核糖核酸酶p的最小底物，在第一个位置的cyUdine最为有效。 A-lacceptor stemvanant比G-lvariant的底物稍好一些，尽管其效率通常明显低于C-lduplex。这在质量上与相应核苷酸的出现频率一致。位置一林的天然底物，对嘧啶最高，对G最低。同位受体茎的核磁共振分析表明，核苷酸位置1处的构象与核糖核酸酶P的底物偏好相关：而C-1采用糖苷角表征的构象在反范围内（接近高GMi），G -1明显处于同步形式在所有情况下，核苷酸的核糖都主要分布在2'-内基上。

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