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Sructure of an RNA Hairpin from HRV-14

机译：HRV-14 RNA发夹的结构

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摘要

The 5' noncoding region of the picomaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem -loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop.
机译：微小病毒基因组的5'非编码区以三叶草开始，这是病毒复制所必需的，至少部分是由于与病毒RNA聚合酶的相互作用，这是与主要病毒蛋白酶融合的一部分。苜蓿叶的必要区域先前已缩小为高度保守的茎环。本文介绍了14个核苷酸的RNA发夹的溶液结构，该结构是人鼻病毒同种型14保守的茎环的一部分。发夹的二级结构与预测的相同：一个五碱基对的茎被序列为UAU的三环束缚。然而，三环的折叠是新颖的，第二环的基部堆叠在茎的闭合基对上，并且与A形几何形状的偏差被引入到毗邻三环的茎区域中，特别是在3'侧。与完整茎环中的第二个顺序相似的茎相比，这些偏差和相关的三环结构可以帮助解释发夹茎区域观察到的独特序列保守性和突变分析数据。

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