...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Characterizationof a 95 kDa High Affinity Human High Density Lipoprotein-Binding Protein
【24h】

Characterizationof a 95 kDa High Affinity Human High Density Lipoprotein-Binding Protein

机译：95 kDa高亲和力人类高密度脂蛋白结合蛋白的表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new human 95 kDa high density lipoprotein (HDL)-binding protein (HBP) corresponding to a high affinity HDL-binding site with Kd = 1.67 fig/mE and a capacity of 13.4 ng/mg was identified in human fetal hepatocytes. The HDL binding with the 95 kDa HBP plateaus at 2.5—5 uglmL under reducing and nonreducing conditions. The association of HDL3 with the 95 kDa HBP plateaued in 15— 30 mm while dissociation was complete in 30 mm. HDL3, apoA-I, and apoA-II were recognized by the95 kDa HBP while low density lipoproteins (LDL) and tetranitromethane-modified HDL were not. The95 kDa HBP predominantly resides on the surface of cells since trypsin treatment of HepG2 cells eliminated nearly 70% of HDL binding. All studied human cells and cell lines (HepG2, Caco-2, HeLa, fibroblasts, SKOV-3, PA-I) demonstrated the presence of the 95 kDa protein. Both RT-PCR and Western blotting for HB-2/ALCAM were negative in human fetal hepatocytes while Gp96IGRP94 was clearly differentiated from the 95 kDa HBP by two-dimensional electrophoretic mobility. Moreover, deglycosylation of HepG2 membrane preparations did not affect either HDL binding to the 95 kDa HBP or its size, while in contrast it affected the molecular weights of HB-2/ALCAM and SR-BIICLA- 1. We conclude that the 95 kDa HBP is a new HDL receptor candidate widely expressed in human cells and cell lines.
机译：在人类胎儿肝细胞中鉴定出一种新的人类95 kDa高密度脂蛋白（HDL）结合蛋白（HBP），其对应于Kd = 1.67 fig / mE和容量13.4 ng / mg的高亲和力HDL结合位点。在还原和非还原条件下，HDL在2.5-5uglmL下与95​​ kDa HBP平台结合。 HDL3与95 kDa HBP的缔合在15-30 mm内达到稳定，而解离在30 mm内完成。 HDL3，apoA-I和apoA-II被95 kDa HBP识别，而低密度脂蛋白（LDL）和四硝基甲烷修饰的HDL则未被识别。由于胰蛋白酶处理的HepG2细胞消除了将近70％的HDL结合，因此95 kDa的HBP主要存在于细胞表面。所有研究的人类细胞和细胞系（HepG2，Caco-2，HeLa，成纤维细胞，SKOV-3，PA-1）均证明存在95 kDa蛋白。 HB-2 / ALCAM的RT-PCR和Western印迹在人胎儿肝细胞中均为阴性，而Gp96IGRP94通过二维电泳迁移率明显不同于95 kDa HBP。此外，HepG2膜制剂的去糖基化作用既不影响HDL与95 kDa HBP的结合，也不影响其大小，相反，它影响HB-2 / ALCAM和SR-BIICLA-1的分子量。我们得出结论，95 kDa HBP是在人类细胞和细胞系中广泛表达的新型HDL受体候选物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号