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首页> 外文期刊>Biochemistry >Translational properties of mHNA, a messenger RNA containing anhydrohexitol nucleotides
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Translational properties of mHNA, a messenger RNA containing anhydrohexitol nucleotides

机译：mHNA的翻译特性，mHNA是含有脱水己糖醇核苷酸的信使RNA

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摘要

Short messenger RNAs (mRNAs) with hexitol residues in two codons were constructed and their properties were studied in an Escherichia coli in vitro translation system. The replacement of the natural ribonucleotides of mRNA in the AUG start codon and the UUC second codon by hexitol nucleotides did not influence the main steps of translation, as indicated by the same level of binding of mRNA with or without hexitol residues under P-site conditions, and the same yield of tRNA binding to the P- and A-sites. Moreover, both peptide formation and translocation took place on mRNAs with hexitol residues. The presence of an A-type messenger hexitol nucleic acid (mHNA)-transfer RNA (tRNA) duplex is important for efficient translation and the 2'-OH function in mRNA is not necessary for binding and movement through the ribosome. Groove shape recognition of the codon-anticodon complex, more than hydrogen-bond interactions of ribose residues in mRNA, is an important factor for correct translation.
机译：构建了带有两个密码子的己糖醇残基的短信使RNA（mRNA），并在大肠杆菌体外翻译系统中研究了它们的特性。用己糖醇核苷酸替换AUG起始密码子和UUC第二个密码子中的mRNA的天然核糖核苷酸不会影响翻译的主要步骤，正如在P位条件下含或不含己糖醇残基的mRNA的结合水平相同，并获得与P和A位点相同的tRNA产量。此外，肽的形成和易位都发生在带有己糖醇残基的mRNA上。 A型信使己糖醇核酸（mHNA）-转移RNA（tRNA）双链体的存在对于有效翻译非常重要，而mRNA中的2'-OH功能对于通过核糖体的结合和移动不是必需的。密码子-反密码子复合物的沟槽形状识别，不仅仅是mRNA中核糖残基的氢键相互作用，是正确翻译的重要因素。

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