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A Novel PCNA-Binding Motif Identified by the Panning of a Random Peptide Display Library

机译：通过淘选随机肽展示文库鉴定的新型PCNA结合基序。

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摘要

Proliferating cell nuclear antigen (PCNA) has recently beenidentified as a target for the binding of proteins involved in DNA replication, DNA repair, and cell cycle control. The interactions between PCNA and a number of these proteins are known to be mediated by a conserved peptide motif. Inthis study, a random peptide library in which peptide sequences are displayed on the E. coli bacterial flagellin protein was sceened for PCNA-binding sequences. Analysis of the retrieved peptide sequence verified the presence of the known PCNA-binding motif. In addition, a second, larger group of peptides containing a different consensus sequence for PCNA binding was discovered. This sequence was found to be present on DNA polymerase #delta#, and a peptide conforming to this sequence was demonstrated to bind to PCNA. Database search and analysis show that many proteins contain the second consensus sequence. These include proteins that are involved in DNA replication, repair, and cell cycle control. The demonstration of this second PCNA-binding motif may provide a basis for identifying and experimentally testing specific proteins for the structural basis for PCNA binding.
机译：近来，增殖细胞核抗原（PCNA）被鉴定为结合DNA复制，DNA修复和细胞周期控制的蛋白质的靶标。已知PCNA与许多这些蛋白质之间的相互作用是由保守的肽基序介导的。在这项研究中，选择了随机肽库，其中肽序列显示在大肠杆菌细菌鞭毛蛋白上，用于PCNA结合序列。检索到的肽序列的分析证实了已知的PCNA结合基序的存在。另外，发现第二组较大的肽，其包含用于PCNA结合的不同共有序列。发现该序列存在于DNA聚合酶#delta上，并且证实符合该序列的肽与PCNA结合。数据库搜索和分析表明，许多蛋白质包含第二个共有序列。这些包括与DNA复制，修复和细胞周期控制有关的蛋白质。第二种PCNA结合基序的演示可以为鉴定和实验测试特定蛋白质作为PCNA结合的结构基础提供基础。

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