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首页> 外文期刊>Biochemistry >Spectral Substructure and Excitonic Interactions in the Minor Photosystem II Antenna Complex CP29 As Revealed by Nonlinear Polarization Spectroscopy in the Frequency Domain
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Spectral Substructure and Excitonic Interactions in the Minor Photosystem II Antenna Complex CP29 As Revealed by Nonlinear Polarization Spectroscopy in the Frequency Domain

机译：非线性极化光谱在频域中揭示的小光系统II天线复合体CP29中的光谱子结构和激子相互作用

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摘要

CP29 (the lhc64 gene product), a minor photosystem II antenna complex, binds six chlorophyll (Chl) a, two Chl b, and two to three xanthophyll molecules. The Chl a/b Q_y absorption band substructure of CP29 (purified from spinach) was investigated by nonlinear polarization spectroscopy in the frequency domain (NLPF) at room temperature. A set of NLPF spectra was obtained at 11 probe wavelengths. Seven probe wavelengths were located in the Q_y spectral region (between 630 and 690 nm) and four in the Soret band (between 450 and 485 nm). Evaluation of the experimental data within the framework of global analysis leads to the following conclusions: (i) The dominant Chl a absorption (with a maximum at 674 nm) splits into (at least) three subbans (centered at 660,670, and 681.5 nm). (ii) In the Chl b region two subbands can be identified with maxima located at 640 and 646 nm. (iii) The lowest energy Q_y transition (peaking at 681.5 nm) is assigned to a Chl a which only weakly interacts with other Chl a or b molecules by incoherent Forster-type excitation energy transfer. (iv) Pronounced excitonic interaction exists between certain Chl a and Chl b molecules, which most likely form a Chl a/b heterodimer. The subbands centered at 640 and 670 nm constitute a strongly coupled Chl a/b pair. The findings of the study indicate that the currently favored view of spectral heterogeneity in CP29 being due essentially to pigment-protein interactions has to be revised.
机译：CP29（lhc64基因产物）是一种次要的光系统II天线复合体，结合了六个叶绿素（Chl）a，两个Chl b和两个至三个叶黄素分子。在室温下，通过频域（NLPF）的非线性偏振光谱研究了CP29（从菠菜中纯化）的Chl a / b Q_y吸收带亚结构。在11个探针波长下获得了一组NLPF光谱。七个探针波长位于Q_y光谱区域（介于630和690 nm之间），四个位于Soret波段（介于450和485 nm之间）。在整体分析框架内对实验数据进行评估得出以下结论：（i）主要的Chla吸收（最大值在674 nm）分成（至少）三个子区（以660,670和681.5 nm为中心） 。 （ii）在Chlb区域中，可以识别两个子带，其最大值位于640和646nm。 （iii）最低能量Q_y跃迁（在681.5 nm处达到峰值）分配给Chla，后者通过非相干的Forster型激发能转移仅与其他Chla或b分子弱相互作用。 （iv）某些Chla和Chlb分子之间存在明显的激子相互作用，这很可能形成Chla a / b异二聚体。以640和670 nm为中心的子带构成了一个强耦合的Chla / b对。该研究的发现表明，目前对CP29中光谱异质性的主要观点主要是由于色素-蛋白质相互作用而引起的，因此必须进行修改。

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