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首页> 外文期刊>Biochemistry >Translocation of Branched-Chain Arginine Peptides through Cell Membranes: Flexibility in the Spatial Disposition of Positive Charges in Membrane-Permeable Peptides
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Translocation of Branched-Chain Arginine Peptides through Cell Membranes: Flexibility in the Spatial Disposition of Positive Charges in Membrane-Permeable Peptides

机译：分支链精氨酸肽通过细胞膜的易位：膜通透性肽中正电荷的空间分布的灵活性。

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摘要

A basic peptide derived from HIV-1 Tat has been reported to have the ability to translocate through cell membranes and to bring exogenous proteins into cells. We have demonstrated that these features could be observed among many arginine-rich peptides, and the presence of a ubiuqitous internalization mechanism for arginine-rich oligopeptides has been suggested. In this report, we report that these features are also applicable to the peptides having branched-chain structures. Peptides that hae arginie residues on four branched chains (R_n)_4 [n (number of arginine residues) = 0-6] were prepared. Fluorescence microscopic observation revealed that the (R_2)_4 peptide exhibited the most efficient translocation. The dependence on the number of arginine residues of the translocation efficiency and cellular localization was also observed for the branched-chain peptides as was seen in the linear peptides. Quite interestingly, efficient translocation was also recognized in the (RG_3R)_4 peptide, where three glycine residues intervened between two arginine residues on each chain of (R_2)_4. The results strongly suggested that a linear structure was not indispensable for the translocation of arginine-rich peptides and that there could be considerable flexibility in the location of the arginine residue in the molecules.
机译：据报道，源自HIV-1 Tat的碱性肽具有转运通过细胞膜并将外源蛋白质带入细胞的能力。我们已经证明可以在许多富含精氨酸的肽中观察到这些特征，并且已经提出了对于富含精氨酸的寡肽存在泛在内在化机制的建议。在本报告中，我们报告这些特征也适用于具有支链结构的肽。制备在四个支链上具有精氨酸残基的肽（R_n）_4 [n（精氨酸残基数）＝ 0-6]。荧光显微镜观察表明，（R_2）_4肽表现出最有效的易位。如在线性肽中所见，对于支链肽，还观察到精氨酸残基数目对转运效率和细胞定位的依赖性。非常有趣的是，在（RG_3R）_4肽中也发现了有效的易位，其中（R_2）_4的每条链上的三个精氨酸残基之间插入了三个甘氨酸残基。结果强烈表明线性结构对于富含精氨酸的肽的转运不是必不可少的，并且精氨酸残基在分子中的位置可能具有相当大的灵活性。

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