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首页> 外文期刊>Biochemistry >Identification of a Thiosulfate Utilization Gene Cluster from the Green Phototrophic Bacterium Chlorobium limicola
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Identification of a Thiosulfate Utilization Gene Cluster from the Green Phototrophic Bacterium Chlorobium limicola

机译：从绿色光养细菌小球藻的硫代硫酸盐利用基因簇的鉴定。

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Chlorobium is an autotrophic, green phototrophic bacterium which uses reduced sulfur compounds to fix carbon dioxide in the light. The pathways for the oxidation of sulfide, sulfur, and thiosulfate have not been characterized with certainty for any species of bacteria. However, soluble cytochrome c-551 and flavocytochrome c (FCSD) have previously been implicated in the oxidation of thiosulfate and sulfide on the basis of enzyme assays in Chlorobium. We have now made a number of observation relating to the oxidation of reduced sulfur compounds. (1) Western analysis shows that soluble cytochrome c-551 in Chlorobium limicola is regulated by thiosulfate, consistent with a role in the utilization of thiosulfate. (2)A membrane-bound flavocytochrome c-sulfide dehydrogenase (which is normally a soluble protein in other species) is constitutive and not regulated by sulfide as expected for an obligately autotrophic species dependent upon sulfide. (3) We have cloned the cytochrome c-551 gene from C. limicola and have found seven other genes, which are also presumably involved in sulfur metabolism and located near that for cytochrome c-551 (SoxA). These include genes for a flavocytochrome c flavoprotein homologue (SoxF2), a nucleotidase homologue (SoxA). These include genes for a flavocytochrome c flavoprotein homologue (SoxF2), a nucleotidase homologue (SoxB), four small proteins (including SoxX, SoxY, and SoxZ), and a thiol-disulfide interchange protein homologue (SowW). (4)We have established that the constitutiely expressed FCSD genes (soxEF1) are located elsewhere in the genome. (5)Through a database search, we have found that the eight thiosulfate utilization genes are clustered in the same order in the Chlorobium tepidum genome (www.tigr.org).Similar thiosulfate utilization gene clusters occur in at least six other bacterial species buty may additionally include genes for rhodanese and sulfite dehydrogenase.
机译：绿毒是一种自养的绿色光养细菌，它使用还原的硫化合物将二氧化碳固定在光下。对于任何种类的细菌，硫化物，硫和硫代硫酸盐的氧化途径尚未确定。但是，基于氯盐中的酶分析，以前已经将可溶性细胞色素c-551和黄细胞色素c（FCSD）参与了硫代硫酸盐和硫化物的氧化。现在，我们对还原的硫化合物的氧化进行了许多观察。 （1）Western分析表明，小球藻中的可溶性细胞色素c-551受硫代硫酸盐调节，这与硫代硫酸盐的利用有关。 （2）膜结合的黄细胞色素c-硫化物脱氢酶（通常是其他物种中的可溶性蛋白）是组成性的，不受硫化物的调节，这是对依赖于硫化物的专性自养物种的预期。 （3）我们从小球藻中克隆了细胞色素c-551基因，并发现了其他七个基因，这些基因可能也参与了硫代谢，并且位于细胞色素c-551（SoxA）的附近。这些包括黄素细胞色素C黄素蛋白同源物（SoxF2），核苷酸酶同源物（SoxA）的基因。这些包括黄素细胞色素C黄素蛋白同源物（SoxF2），核苷酸酶同源物（SoxB），四个小蛋白（包括SoxX，SoxY和SoxZ）和硫醇-二硫键互换蛋白同源物（SowW）的基因。 （4）我们已经确定，组成型表达的FCSD基因（soxEF1）位于基因组的其他位置。 （5）通过数据库搜索，我们发现了8个硫代硫酸盐利用基因以相同的顺序聚集在绿球藻（Chlorobium tepidum）基因组中（www.tigr.org）。相似的硫代硫酸盐利用基因簇还存在于至少六个其他细菌物种中可能还包括罗丹酶和亚硫酸盐脱氢酶的基因。

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