A critical residue in the folding pathway of an integral membrane protein.

Nagy JK; Sanders CR

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A critical residue in the folding pathway of an integral membrane protein.

机译：完整膜蛋白折叠途径中的关键残基。

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Although a number of common diseases are a direct consequence of membrane protein misfolding, studies of membrane protein folding and misfolding lag well behind those of soluble proteins. Here it is shown that an interfacial residue, Tyr16, of the integral membrane protein diacylglycerol kinase (DAGK) plays a critical role in the folding pathway of this protein. Properly folded Y16C exhibits kinetic parameters and stability similar to wild-type DAGK. However, when unfolded and then allowed to spontaneously fold in the presence of model membranes, Y16C exhibits dramatically lower rates and efficiencies of functional assembly compared to the wild-type protein. The Y16C mutant represents a class of mutations which may be commonly found in disease-related membrane proteins.

机译：尽管许多常见疾病是膜蛋白错误折叠的直接结果，但对膜蛋白折叠和错误折叠的研究却远远落后于可溶性蛋白。此处显示完整膜蛋白二酰基甘油激酶（DAGK）的界面残基Tyr16在该蛋白的折叠途径中起关键作用。正确折叠的Y16C具有类似于野生型DAGK的动力学参数和稳定性。但是，当展开并随后在模型膜存在下自发折叠时，与野生型蛋白相比，Y16C的功能组装效率和效率大大降低。 Y16C突变体代表一类可能在疾病相关膜蛋白中常见的突变。

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《Biochemistry》 |2002年第29期|共5页
作者
Nagy JK; Sanders CR;
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关键词
Membrane Proteins; mutant; Assault by stabbing; 1; 2-diacylglycerol; 膜蛋白质类;

机译：Membrane Proteins;mutant;Assault by stabbing;1;2-diacylglycerol;膜蛋白质类;

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1. A critical residue in the folding pathway of an integral membrane protein. [J] . Nagy JK, Sanders CR Biochemistry . 2002,第29期

机译：完整膜蛋白折叠途径中的关键残基。
2. Possible locally driven folding pathways of TC5b, a 20-residue protein. [J] . Nikiforovich GV, Andersen NH, Fesinmeyer RM, Proteins: Structure, Function, and Genetics . 2003,第2期

机译：TC5b（20个残基的蛋白质）的可能局部驱动的折叠途径。
3. The contribution of a covalently bound cofactor to the folding and thermodynamic stability of an integral membrane protein. [J] . Curnow P, Booth PJ Journal of Molecular Biology . 2010,第4期

机译：共价结合的辅因子对完整膜蛋白的折叠和热力学稳定性的贡献。
4. Helical net plots and surface lipophilicity mapping of transmembrane helices of integral membrane proteins:aids to 3D structure determination of integral membrane proteins [C] . Mire Zloh, Diego Esposito, William A.Gibbons the International Peptide Symposium . 2001

机译：整体膜蛋白透气纸螺旋螺旋网图和表面亲脂性测定：有助于3D结构测定整体膜蛋白的测定
5. A study of the structure, folding and assembly of diacylglycerol kinase, an integral membrane protein. [D] . Nagy, Joanna Kathleen. 2002

机译：研究二酰基甘油激酶（一种完整的膜蛋白）的结构，折叠和组装。
6. The human erythrocyte anion-transport protein. Further amino acid sequence from the integral membrane domain homologous with the murine protein. [O] . C J Brock, M J Tanner 1986

机译：人红细胞阴离子转运蛋白。来自完整膜结构域的与鼠蛋白同源的其他氨基酸序列。
7. The human erythrocyte anion-transport protein. Further amino acid sequence from the integral membrane domain homologous with the murine protein. [O] . Brock, C J, Tanner, M J 1986

机译：人红细胞阴离子转运蛋白。来自完整膜结构域的与鼠蛋白同源的其他氨基酸序列。
8. Evidence that Eel Acetylcholinesterase Is Not an Integral Membrane Protein. [R] . Millar, D. B., Christopher, J. P., Burrough, D. O. 1978

机译：鳗乙酰胆碱酯酶不是整合膜蛋白的证据。

A critical residue in the folding pathway of an integral membrane protein.

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