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首页> 外文期刊>Biochemistry >Characterization of glycosylation sites of the epidermal growth factor receptor.
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Characterization of glycosylation sites of the epidermal growth factor receptor.

机译：表皮生长因子受体糖基化位点的表征。

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摘要

The epidermal growth factor receptor is a transmembrane glycoprotein that mediates the cellular responses to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). In this study of the human EGF receptor naturally expressed in A431 cells, the glycosylation sites of the full-length, membrane-bound receptor and of a secreted form of the receptor were characterized by mass spectrometry. Our data show that the naturally expressed human EGF receptor is fully glycosylated on eight of the 11 canonical sites; two of the sites are not glycosylated, and one is partially glycosylated, a pattern of site-usage similar but not identical to those reported for the recombinant human EGF receptor heterologously expressed in Chinese hamster ovary cells. We also confirm the partial glycosylation of an atypical NNC site first identified in the receptor expressed in Chinese hamster ovary cells. We show that an additional canonical site in the secreted form of the receptor is fully glycosylated. While the pattern of glycosylation is the same for the sites shared by the full-length and the secreted forms of the receptor, the oligosaccharides of the full-length receptor are more extensively processed. Finally, we provide evidence that in addition to the known secreted form of the receptor, a proteolytic cleavage product of the receptor corresponding to the full extracytoplasmic, ligand-binding domain is present in the conditioned medium.
机译：表皮生长因子受体是一种跨膜糖蛋白，可介导细胞对表皮生长因子（EGF）和转化生长因子-α（TGF-alpha）的反应。在对自然在A431细胞中表达的人EGF受体的研究中，通过质谱分析了全长，膜结合受体和受体的分泌形式的糖基化位点。我们的数据表明，自然表达的人EGF受体在11个规范位点中的8个位点完全被糖基化。其中两个位点未糖基化，一个位点被部分糖基化，这种位点使用模式与报道的在中国仓鼠卵巢细胞中异源表达的重组人EGF受体相似，但并不完全相同。我们还确认了非典型NNC位点的部分糖基化，该位点首先在中国仓鼠卵巢细胞表达的受体中鉴定。我们显示，受体的分泌形式中的另一个规范位点被完全糖基化。虽然受体的全长和分泌形式共有的位点的糖基化方式相同，但全长受体的寡糖却得到了更广泛的加工。最后，我们提供证据表明，除了已知的受体分泌形式外，条件培养基中还存在对应于完整胞质外配体结合结构域的受体蛋白水解切割产物。

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