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首页> 外文期刊>Biochemistry >Ranacyclins,a new Family of Short Cyclic Antimicrobial Peptides:Biological Function,Mode of Action,and Parameters Involved in Target Specificity
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Ranacyclins,a new Family of Short Cyclic Antimicrobial Peptides:Biological Function,Mode of Action,and Parameters Involved in Target Specificity

机译：Ranacyclins，短周期抗菌肽的一个新家族：生物学功能，作用方式和涉及目标特异性的参数。

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摘要

We report on two new cyclic 17-residue peptides that we named ranacyclins E and T,the first isolated from Rana esculenta frog skin secretions and the second discovered by screening a cDNA library from Rana temporaria.Ranacyclins have a loop region that is homologous with that of an 18-mer peptide,pLR,isolated from the skin of the Northern Leopard frog,Rana pipiens,with no reported antimicrobial activity.Here we show that ranacyclins and plR have antimicrobial and antifungal activity.However,despite the high structural similarity,they differ in their spectrum of activity.The data reveal that ranacyclins and pLR have several properties thatdifferentiate them from most known antimicrobial peptides.These include the following: (i) they adopt a significant portion of random coil structure in the membrane as revealed by ATR-FTIR and CD spectroscopy (50% for rancyclin T and 70% for both ranacyclin E and plR);(ii) they bind similarly to both zwitterionic and negatively charged membranes as revealed by using tryptophan fluorescence and surfaxce plasmon resonance (SPR;BiAcore biosensor);(iii) they insert into the hydrophobic core of the membrane and presumably form transmembrane pores without damage to the bacterial wall,as revealed by SPR,ATR-FTIR,and transmission electron microscopy (TEM); and (iv) despite being highly and equally active in permeating bacterial spheroplasts and negatively charged membranes,they differ significantly in their potencies against target cells.Furthermore,a significantly fraction of a given secondary structure is not prerequisite for membrane permeation and antimicrobial activity.However,increasing the fraction of a secondary structure and reducing peptide assembly in the membrane make it easier for the peptide to diffuse through the cell wall,which is different for each microorganism,into the cytoplasmic membrane.
机译：我们报道了两个新的环状17残基肽，我们将其分别命名为ranacyclins E和T，第一个是从蓝蛙蛙皮肤分泌物中分离出来的，第二个是通过筛查了Rana temporaria的cDNA文库发现的.Ranacyclins具有一个与之同源的环区从北豹蛙蛙皮的皮肤中分离出的一种18-mer肽pLR，未见抗菌活性。在这里，我们显示了ranacyclins和plR具有抗菌和抗真菌活性。然而，尽管结构相似性很高，数据显示，雷纳环素和pLR具有使其与大多数已知抗菌肽区分开的几种特性，这些特性包括：（i）它们在膜中采用了很大一部分随机卷曲结构，如ATR- FTIR和CD光谱（rancyclin T的50％和ranacyclin E和plR的70％）;（ii）它们与两性离子膜和带负电荷的膜相似地结合为rev通过色氨酸荧光和表面等离子体激元共振（SPR; BiAcore生物传感器）进行密封;（iii）它们插入膜的疏水核中并形成跨膜孔，而不会损坏细菌壁，如SPR，ATR-FTIR和透射电子显微镜（TEM）; （iv）尽管在渗透细菌原生质球和带负电的膜中具有同等的活性，但它们对靶细胞的效力却有显着差异。此外，给定二级结构的很大一部分并不是膜渗透和抗菌活性的先决条件。 ，增加二级结构的比例并减少膜中的肽组装，使肽更容易扩散通过细胞壁，每种微生物都不同，进入细胞质膜。

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