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首页> 外文期刊>Biochemistry >Oligodeoxynucleotides Having a Loop Consisting of 3'-Deoxy-4'-C-(2-hydroxyethyl)thymidines Form Stable Hairpins
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Oligodeoxynucleotides Having a Loop Consisting of 3'-Deoxy-4'-C-(2-hydroxyethyl)thymidines Form Stable Hairpins

机译：具有由3'-脱氧-4'-C-（2-羟乙基）胸苷组成的环的寡脱氧核苷酸形成稳定的发夹

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摘要

Components that form stable hairpin loops are highly useful for the development of functional DNA and RNA molecules.We have designed and synthesized a sugar-modified thymidine analogue,3'-deoxy-4'-C-(2-hydroxyethyl)thymidine (X),as a nucleosidic loop component stabilizing the hairpin structure.The ODNs 1-1-4,5'-d[CGAACG-X_n-CGTTCG]-3' (1-1,n=1;I-2,n=2;I-3,n=3;I-4,n=4),forming the hairpin loop structures,of which the loop moiety consisted of the analogue X,and also the corresponding unmodified ODNs II-1-4,5'-d[CGAACG-T_n-CGTTCG]-3' (II-1,n=1;II-2,n=2;II-3,n=3;II-4,n=4),having a thymidine loop,were synthesized by the phosphoramidite method.The melting temperatures (T_m) of the ODNs 1-1-4 containing X in the loop moiety at 5 muM were 67.1,68.1,73.0,and 69.3 deg C,respectively,and those of the control natural ODNs II-1-4 were 65.3,67.0,69.2,and 68.8 deg C,respectively.Thus,the ODNs 1-1-4 formed a more thermally stable hairpin than the corresponding unmodified ODNs II-l-4 having an equal number of loop residues.The hairpin structures of the modified ODNs 1-1-4 and the unmodified ODNs II-l-4 were investigated by CD spectroscopy and molecular mechanics calculations.These results showed that the 4'-branched nucleoside X can stabilize hairpin structures when it is present in the loop moiety,probably due to the flexibility of the one-carbon-elongated 4'-branched structure.
机译：形成稳定发夹环的成分对于功能性DNA和RNA分子的开发非常有用。我们设计并合成了糖修饰的胸苷类似物3'-脱氧-4'-C-（2-羟乙基）胸苷（X） ODNs 1-1-4,5'-d [CGAACG-X_n-CGTTCG] -3'（1-1，n = 1; I-2，n = 2;是稳定发夹结构的核苷环成分。 I-3，n ＝ 3; I-4，n ＝ 4），形成发夹环结构，其中的环部分由类似物X以及相应的未修饰的ODN II-1-4,5'-d组成具有胸苷环的[CGAACG-T_n-CGTTCG] -3'（II-1，n = 1; II-2，n = 2; II-3，n = 3; II-4，n = 4）在5μM的环部分中含X的ODN 1-1-4的熔融温度（T_m）与对照天然ODN的熔融温度（T_m）分别为67.1、68.1、73.0和69.3℃。 II-1-4分别为65.3、67.0、69.2和68.8摄氏度。因此，ODN 1-1-4形成了比相应的具有相同nu的未修饰ODN II-1-4更热稳定的发夹。通过CD光谱和分子力学计算研究了修饰的ODNs 1-1-4和未修饰的ODNs II-1-4的发夹结构，结果表明4'-分支核苷X可以稳定发夹结构当其存在于环部分中时，其结构可能是由于一碳长的4'-支化结构的柔性。

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