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首页> 外文期刊>Biochemistry >Expression of Scavenger Receptor BI Facilitates Sterol Movement between the Plasma Membrane and the Endoplasmic Reticulum in Macrophages.
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Expression of Scavenger Receptor BI Facilitates Sterol Movement between the Plasma Membrane and the Endoplasmic Reticulum in Macrophages.

机译：清除剂受体BI的表达促进巨噬细胞中质膜和内质网之间的甾醇移动。

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摘要

Scavenger receptor BI influences multiple aspects of cellular sterol metabolism. In this series of studies, we evaluated the effect of scavenger receptor BI expression on the distribution and movement of sterol between the plasma membrane and the endoplasmic reticulum in macrophages, by comparing control J774 cells to J774 cells in which SR-BI expression was constitutively increased 3-fold. J774 cells with increased expression of SR-BI (J774-SRBI cells) esterified plasma membrane cholesterol more rapidly as compared to control cells. The esterification of endogenously synthesized cholesterol was also more rapid in cells with increased SR-BI expression; this could be partially suppressed by removing cholesterol from the plasma membrane. The increased plasma membrane sterol esterification in J774-SRBI cells was not due to increased acyl-coA:cholesterol acyltransferase activity and was observed even though J774-SRBI cells manifested a smaller free cholesterol pool in the endoplasmic reticulum. Cholesterolester hydrolysis was also more rapid in J774-SRBI cells. Increased expression of SR-BI also facilitated the clearance of cellular cholesterol ester to HDL(3). This latter observation, combined with the measurement of the smaller ER free cholesterol pool in J774-SRBI cells, suggests that the free cholesterol derived from the hydrolysis of cholesterol ester was rapidly transported back to the plasma membrane. It is concluded that expression of SR-BI in macrophages increases the rate of free cholesterol transport, and modulates free cholesterol distribution between the plasma membrane and the internal membrane compartments in macrophages.
机译：清道夫受体BI影响细胞固醇代谢的多个方面。在这一系列研究中，我们通过比较对照J774细胞和SR-BI表达组成性增加的J774细胞，评估了清道夫受体BI表达对巨噬细胞质膜和内质网之间固醇分布和运动的影响。 3倍。与对照细胞相比，SR-BI表达增加的J774细胞（J774-SRBI细胞）酯化了质膜胆固醇。内源性合成胆固醇的酯化在SR-BI表达增加的细胞中也更快。通过从质膜中去除胆固醇可以部分抑制这种情况。 J774-SRBI细胞中质膜固醇酯化的增加不是由于酰基coA：胆固醇酰基转移酶活性的增加，即使J774-SRBI细胞在内质网中表现出较小的游离胆固醇池，也可以观察到。在J774-SRBI细胞中，胆固醇的水解也更快。 SR-BI表达的增加也促进了细胞胆固醇酯向HDL的清除（3）。后一项观察结果与对J774-SRBI细胞中较小的ER游离胆固醇库的测量相结合，表明源自胆固醇酯水解的游离胆固醇被迅速转运回质膜。结论是，SR-BI在巨噬细胞中的表达增加了游离胆固醇运输的速率，并调节了巨噬细胞质膜和内膜区室之间的游离胆固醇分布。

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