Inhibition of Protein Interactions with the beta_2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from beta_2-Binding Proteins

Gene Wijffels; Brian P.Dalrymple; Pavel Prosselkov; Kritaya Kongsuwan; V.Chandana Epa; Penelope E.Lilley; Slobodan Jergic; Jens Buchardt; Susan E.Brown; Paul F.Alewood; Philip A.Jennings; Nicholas E.Dixon

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Inhibition of Protein Interactions with the beta_2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from beta_2-Binding Proteins

机译：β_2结合蛋白的肽对大肠杆菌DNA聚合酶III的beta_2滑动夹的蛋白质相互作用的抑制作用

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The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair.A universal interaction motif is proposed to be one mechanism by which those proteins bind the E.coli sliding clamp,a homodimer of the beta subunit,at a single site on the dimer.The numerous beta_2-binding proteins have various versions of the consensus interaction motif,including a related hexameric sequence.To determine if the variants of the motif could contribute to the competition of the beta-binding proteins for the beta_2 site,synthetic peptides derived from the putative beta_2-binding motifs were assessed for their abilities to inhibit protein-beta_2 interactions,to bind directly to beta_2,and to inhibit DNA synthesis in vitro.A hierarchy emerged,which was consistent with sequence similarity to the pentameric consensus motif,QL(S/D)LF,and peptides containing proposed hexameric motifs were shown to have activities comparable to those containing the consensus sequence.The hierarchy of peptide binding may be indicative of a competitive hierarchy for the binding of proteins to beta_2 in various stages or circumstances of DNA replication and repair.

机译：现已理解，大肠杆菌复制体的滑动夹具可与许多参与DNA合成和修复的蛋白质相互作用。普遍的相互作用基序被认为是这些蛋白质结合E.coli滑动夹具（β的同二聚体）的一种机制。大量的beta_2结合蛋白具有多种版本的共有相互作用基序，包括相关的六聚体序列。确定该基序的变体是否有助于促进β结合蛋白的竞争对于beta_2位点，评估了推定的beta_2结合基序衍生的合成肽抑制蛋白质-beta_2相互作用，直接与beta_2结合以及体外抑制DNA合成的能力。出现了层次结构，该序列与序列一致与五聚体共有基序QL（S / D）LF具有相似性，并且含有拟议六聚体基序的肽具有与含五聚体共有基序相当的活性肽结合的层次可以指示在DNA复制和修复的不同阶段或情况下蛋白质与β_2结合的竞争层次。

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《Biochemistry》 |2004年第19期|共11页
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Gene Wijffels; Brian P.Dalrymple; Pavel Prosselkov; Kritaya Kongsuwan; V.Chandana Epa; Penelope E.Lilley; Slobodan Jergic; Jens Buchardt; Susan E.Brown; Paul F.Alewood; Philip A.Jennings; Nicholas E.Dixon;
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1. Inhibition of Protein Interactions with the beta_2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from beta_2-Binding Proteins [J] . Gene Wijffels, Brian P.Dalrymple, Pavel Prosselkov, Biochemistry . 2004,第19期

机译：β_2结合蛋白的肽对大肠杆菌DNA聚合酶III的beta_2滑动夹的蛋白质相互作用的抑制作用
2. Sliding clamp-DNA interactions are required for viability and contribute to DNA polymerase management in Escherichia coli. [J] . Heltzel JM, Scouten-Ponticelli SK, Sanders LH, Journal of Molecular Biology . 2009,第1期

机译：滑动钳位DNA相互作用是生存所需的，并有助于大肠杆菌中DNA聚合酶的管理。
3. The Plasmid RK2 Replication Initiator Protein (TrfA) Binds to the Sliding Clamp β Subunit of DNA Polymerase III: Implication for the Toxicity of a Peptide Derived from the Amino-Terminal Portion of 33-Kilodalton TrfA [J] . Kritaya Kongsuwan, Peter Josh, Marc J. Picault, Journal of bacteriology . 2006,第15期

机译：质粒RK2复制起始蛋白（TrfA）绑定到DNA聚合酶III的滑动夹β亚基：对33千达尔顿TrfA氨基末端部分衍生的肽的毒性的影响。
4. Towards an understanding of protein-protein interaction network hierarchies. Analysis of DnaN (β)-binding peptide motifs in members of protein families interacting with the eubacterial processivity clamp, the β subunit of DNA Polymerase III [C] . Brian P. Dalrymple, Gene Wijffels, Kritaya Kongsuwan, Asia-Pacific Bioinformatics Conference(APBC 2003); 200302; Adelaide(AU) . 2003

机译：对蛋白质-蛋白质相互作用网络层次结构的理解。蛋白质家族成员与DNA聚合酶III的优生过程性钳位相互作用的DnaN（β）结合肽基序分析
5. Dynamic protein-protein and protein-DNA interactions within the Escherichia coli DNA polymerase III sliding clamp loader. [D] . Anderson, Stephen Gregory. 2008

机译：大肠杆菌DNA聚合酶III滑动钳式装载器内的动态蛋白质-蛋白质和蛋白质-DNA相互作用。
6. Collision with duplex DNA renders Escherichia coli DNA polymerase III holoenzyme susceptible to DNA polymerase IV-mediated polymerase switching on the sliding clamp [O] . Thanh Thi Le, Asako Furukohri, Masahiro Tatsumi-Akiyama, -1

机译：与双链DNA的碰撞使大肠杆菌DNA聚合酶III全酶易受DNA聚合酶IV介导的聚合酶在滑动钳上转换的影响
7. Inhibition of Protein Interactions with the b2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from b2-Binding Proteins [O] . -1

机译：通过B2结合蛋白的肽抑制与大肠杆菌大肠杆菌DNA聚合酶III的B2滑动钳位的蛋白质相互作用

Inhibition of Protein Interactions with the beta_2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from beta_2-Binding Proteins

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