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首页> 外文期刊>Biochemistry >Single Live Cell Imaging of Chromosomes in Chloramphenicol-Induced Filamentous Pseudomonas aeruginosa
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Single Live Cell Imaging of Chromosomes in Chloramphenicol-Induced Filamentous Pseudomonas aeruginosa

机译：氯霉素诱导的丝状铜绿假单胞菌中染色体的单活细胞成像

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Pseudomonas aeruginosa is a leading opportunistic pathogen in human infections,and it is renowned for its intrinsic resistance to structurally and functionally unrelated antibiotics.Filamentation induced by antibiotics appears to trigger bacteria to depart from a normal growth phase and enter a stationary growth phase.As antibiotic concentrations decline below a therapeutic range,filamentous bacteria begin growth phase.As antibiotic concentrations decline below a therapeutic range,filamentous bacteria begin to divide normally,leading to more regrowth of the bacteria.Furthermore,filamentous bacteria are associated with an increase in endotoxin release.Moreover,the immune system of a patient needs to cope with uncharacteristic filamentous bacteria.Thus,it is biologically and clinically significant to study and understand bacterial filamentation.In this study,we investigate the frequencies,conditions,and characteristics of a filamentous P.aeruginosa at single cell and single chromosome resolutions.Our results show that filamentous cells (elongated rods) contain multiple copies of the cell's chromosome.It appears that the unsuccessful segregation of replicated chromosomes in an individual cell accompanies the formation of undivided filamentous cells.The quantity of chromosomes and the length of the filamentous wild-type cells increase as the chloramphenicol concentration increases to 50 and 250 mug/mL,suggesting that chloramphenicol induces the filamentation.Filamentation of three strains of P.aeruginosa depends on the expression level of efflux pump (MexAB-OprM) and the minimum inhibitory concentration of chloramphenicol.This study also opens up the new possibility of real-time monitoring of modes of actions of antibiotics in live cells with both temporal and spatial resolution.
机译：铜绿假单胞菌（Pseudomonas aeruginosa）是人类感染中的主要机会病原体，以其对结构和功能上不相关的抗生素的内在抗性而闻名。抗生素诱导的长丝似乎触发细菌脱离正常生长期并进入静止生长期。当抗生素浓度下降到治疗范围以下时，丝状细菌开始生长期。当抗生素浓度下降到治疗范围以下时，丝状细菌开始正常分裂，导致更多的细菌再生。此外，丝状细菌还与内毒素释放增加有关。此外，患者的免疫系统需要应对不典型的丝状细菌。因此，研究和了解细菌丝化在生物学和临床上具有重要意义。在本研究中，我们研究了铜绿假单胞菌的频率，状况和特征。在单个细胞和单个染色体上结果表明，丝状细胞（伸长的杆）包含细胞染色体的多个副本，似乎单个细胞中复制染色体的不成功分离伴随着未分裂的丝状细胞的形成，染色体的数量和长度随着氯霉素浓度增加至50和250杯/ mL，丝状野生型细胞增加，这表明氯霉素诱导了丝化。三株绿脓杆菌的细丝化取决于外排泵的表达水平（MexAB-OprM）和最小氯霉素的抑制浓度。这项研究也为实时监测空间和时间分辨率下活细胞中抗生素的作用方式提供了新的可能性。

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