Covalent binding and interstrand cross-linking of duplex DNA by dirhodium(II,II) carboxylate compounds.

Dunham SU; Chifotides HT; Mikulski S; Burr AE; Dunbar KR

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Covalent binding and interstrand cross-linking of duplex DNA by dirhodium(II,II) carboxylate compounds.

机译：二价二羧酸（II，II）羧酸盐化合物对双链DNA的共价结合和链间交联。

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The study of the interactions of double-stranded (ds) DNA with the dirhodium carboxylate compounds Rh(2)(O(2)CCH(3))(4)(H(2)O)(2) (Rh1), [Rh(2)(O(2)CCH(3))(2)(CH(3)CN)(6)](BF(4))(2) (Rh2), and Rh(2)(O(2)CCF(3))(4) (Rh3) supports the presence of covalently linked DNA adducts, including stable DNA interstrand cross-links. The present biochemical study refutes earlier claims that no reaction between dirhodium compounds and dsDNA occurs. The reversal behavior of these interstrand cross-links in 5 M urea at 95 degrees C (for different heating times) implies the presence of various coordination modes involving ax/ax, ax/eq, and eq/eq DNA interactions with the dirhodium core. The reaction rates of the dirhodium compounds with dsDNA were determined spectroscopically and are in the order Rh1 Rh2 < Rh3. This difference in behavior of the three dirhodium compounds correlates with the lability of the leaving groups and corresponds to the extent of interstrand cross-link formation by these compounds on a 123 bp DNA fragment, as observed by denaturing polyacrylamide gel electrophoresis (dPAGE). Since all three dirhodium compounds form covalent Rh-DNA adducts, including interstrand cross-links, it is important that DNA be considered a potential target for biological activity of these dirhodium carboxylate compounds.

机译：研究双链（ds）DNA与羧酸铑的化合物Rh（2）（O（2）CCH（3））（4）（H（2）O）（2）（Rh1）的相互作用，[ Rh（2）（O（2）CCH（3））（2）（CH（3）CN）（6）]（BF（4））（2）（Rh2）和Rh（2）（O（2 ）CCF（3））（4）（Rh3）支持共价连接的DNA加合物的存在，包括稳定的DNA链间交联。目前的生化研究驳斥了先前的说法，即ho化合物与dsDNA之间没有反应。这些链间交联在5 M尿素中在95摄氏度（对于不同的加热时间）的逆转行为表明存在各种配位模式，包括与/核核心的ax / ax，ax / eq和eq / eq DNA相互作用。用光谱法确定二价铑化合物与dsDNA的反应速率，顺序为Rh1 Rh2

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《Biochemistry》 |2005年第3期|共8页
作者
Dunham SU; Chifotides HT; Mikulski S; Burr AE; Dunbar KR;
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正文语种 eng
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DNA; carboxylate; Rhodium; behavioral aspects; 铑;

机译：DNA;羧酸盐;铑;行为特征;极性;

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1. Covalent binding and interstrand cross-linking of duplex DNA by dirhodium(II,II) carboxylate compounds. [J] . Dunham SU, Chifotides HT, Mikulski S, Biochemistry . 2005,第3期

机译：二价二羧酸（II，II）羧酸盐化合物对双链DNA的共价结合和链间交联。
2. The minor groove-binding agent ELB-21 forms multiple interstrand and intrastrand covalent cross-links with duplex DNA and displays potent bactericidal activity against methicillin-resistant Staphylococcus aureus [J] . Helena Rosado, Khondaker M. Rahman, Eva-Anne Feuerbaum, Journal of Antimicrobial Chemotherapy . 2011,第5期

机译：小沟结合剂ELB-21与双链DNA形成多个链间和链内共价交联，并显示出对耐甲氧西林的金黄色葡萄球菌的有效杀菌活性
3. The minor groove-binding agent ELB-21 forms multiple interstrand and intrastrand covalent cross-links with duplex DNA and displays potent bactericidal activity against methicillin-resistant Staphylococcus aureus. [J] . Rosado H, Rahman KM, Feuerbaum EA, The Journal of Antimicrobial Chemotherapy . 2011,第5期

机译：小沟结合剂ELB-21与双链体DNA形成多个链间和链内共价交联，并显示出对耐甲氧西林的金黄色葡萄球菌的有效杀菌活性。
4. The mechanism of base-excision DNA repair by Endonuclease-VIII as revealed from its trapped covalent intermediate with a DNA substrate [C] . Gali Golan, Dmitry O.Zharkov, Rotem Gilboa International Peptide Symposium;European Peptide Symposium . 2005

机译：通过与DNA底物的被捕获的共价中间体揭示的内切核酸酶-VIII碱基切除DNA修复机制
5. I. Multicatalysis: Development of a Bismuth(III) triflate-catalyzed Nucleophilic addition/ Hydrofunctionalization Reaction in the Synthesis of Complex Heterocycles II. C--H Arylation of Arenes and Heteroarenes with Palladium-Carboxylate Catalysts: Identification of the Catalyst Resting State and the Crucial Role of Pivalate Ligand in Stabilization of the Catalyst III. C--H Arylation of Heteroarenes with Palladium-Carboxylate Catalysts: Importance of Phosphine Ligand for Basic Heteroarene Substrates IV. C--H Arylation of Heteroarenes with Palladium-Carboxylate Catalysts: Effect of the Properties of Basic Heteroarene Substrates on Direct Arylations. [D] . Tundel, Rachel E. 2011

机译：I.多催化作用：在复杂的杂环化合物的合成中三氟甲磺酸铋（III）催化的亲核加成/加氢官能化反应的发展。羰基钯催化剂对芳烃和杂芳烃的CH-H芳基化反应：催化剂静止状态的鉴定以及新戊酸酯配体在稳定催化剂中的关键作用III。羰基钯催化剂催化杂芳烃的CH芳基化：膦配体对基础杂芳烃基质的重要性IV。杂芳烃与钯-羧酸酯的CH芳基化：碱性杂芳烃底物性能对直接芳基化的影响。
6. The minor groove-binding agent ELB-21 forms multiple interstrand and intrastrand covalent cross-links with duplex DNA and displays potent bactericidal activity against methicillin-resistant Staphylococcus aureus [O] . Helena Rosado, Khondaker M. Rahman, Eva-Anne Feuerbaum, -1

机译：小沟结合剂ELB-21与双链DNA形成多个链间和链内共价交联并显示出对耐甲氧西林的金黄色葡萄球菌的有效杀菌活性
7. Covalent Binding and Interstrand Cross- Linking of Duplex DNA by Dirhodium (II, II) Carboxylate Compounds [O] . -1

机译：甲酸钠化合物的双相DNA共价结合和交叉链接

Covalent binding and interstrand cross-linking of duplex DNA by dirhodium(II,II) carboxylate compounds.

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