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Thermodynamics of statherin adsorption onto hydroxyapatite

机译：斯大林吸附羟基磷灰石的热力学

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摘要

Statherin is a salivary protein that inhibits the nucleation and growth of hydroxyapatite crystals in the supersaturated environment of the oral cavity. The thermodynamics of adsorption of statherin onto hydroxyapatite crystals have been characterized here by isothermal titration calorimetry and equilibrium adsorption isotherm analysis. At 25 degrees C, statherin adsorption is characterized by an exothermic enthalpy of similar to 3 kcal/mol that diminishes to zero at similar to 25% surface coverage. The initial heat of statherin adsorption increases with temperature, displaying a positive heat capacity change of 194 +/- 7 cal K-1 mol(-1) at 25 degrees C. The heat of adsorption during this initial phase is strongly dependent on the buffer species, and from the differential heats of buffer ionization, it can be calculated that approximately one proton is taken up by the crystal or protein upon adsorption. The free energy of adsorption is dominated at all coverages by a large positive entropy ( >= 23 cal K-1 mol(-1)), which may be partially due to the loss of organized water that hydrates the protein and the mineral surface prior to adsorption. These results are interpreted using a two-site model for adsorption of statherin onto the hydroxyapatite crystals.
机译：Statherin是一种唾液蛋白，可以抑制口腔中过饱和环境中羟磷灰石晶体的形核和生长。此处已通过等温滴定量热法和平衡吸附等温线分析表征了他汀在羟基磷灰石晶体上的吸附热力学。在25摄氏度时，斯坦斯汀吸附的特征是类似于3 kcal / mol的放热焓，在接近25％的表面覆盖率时，该焓降为零。 Statherin吸附的初始热量随温度增加，在25摄氏度下显示正热容变化194 +/- 7 cal K-1 mol（-1）。在此初始阶段，吸附热强烈依赖于缓冲液根据缓冲离子化的不同热量，可以计算出晶体或蛋白质在吸附时吸收了大约一个质子。吸附的自由能在所有范围内都被大的正熵（> = 23 cal K-1 mol（-1））所支配，这可能部分是由于失去了使蛋白质和矿物质表面水合的有组织水去吸附。这些结果使用两点模型来解释，其中所述模型用于将Statherin吸附到羟基磷灰石晶体上。

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