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首页> 外文期刊>Biochemistry >The Drosophila class B scavenger receptor NinaD-I is a cell surface receptor mediating carotenoid transport for visual chromophore synthesis
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The Drosophila class B scavenger receptor NinaD-I is a cell surface receptor mediating carotenoid transport for visual chromophore synthesis

机译：果蝇B类清道夫受体NinaD-I是介导类胡萝卜素转运的细胞表面受体，用于视觉生色团的合成

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The blind Drosophila mutant ninaD lacks the visual chromophore. Genetic evidence that the molecular basis is a defect in carotenoid uptake which causes vitamin A deficiency exists. The ninaD gene encodes a scavenger receptor that is significantly homologous in sequence with the mammalian scavenger receptors SR-BI (scavenger receptor class B type I) and CD36 (cluster determinant 36), yet NinaD has not been characterized in functional detail. Therefore, we established a Drosophila S2 cell culture system for biochemically characterizing the ninaD gene products. We show that the two splice variant isoforms encoded by ninaD exhibit different subcellular localizations. NinaD-I, the long protein variant, is localized at the plasma membrane, whereas the short variant, NinaD-II, is localized at intracellular membranes. Only NinaD-I could mediate the cellular uptake of carotenoids from micelles in this cell culture system. Carotenoid uptake was concentration-dependent and saturable. By in vivo analyses of different mutant and transgenic fly strains, we provide evidence of an essential role of NinaD-I in the absorption of dietary carotenoids to support visual chromophore synthesis. Moreover, our analyses suggest a role of NinaD-I in tocopherol metabolism. Even though Drosophila is a sterol auxotroph, we found no evidence of a contribution of NinaD-I to the uptake of these compounds. Together, our study establishes an evolutionarily conserved connection between class B scavenger receptors and the numerous functions of fat soluble vitamins in animal physiology.
机译：盲果蝇突变体ninaD缺乏视觉发色团。存在分子基础是类胡萝卜素摄取缺陷的遗传证据，该缺陷导致维生素A缺乏。 ninaD基因编码的清道夫受体与哺乳动物的清道夫受体SR-BI（B类清道夫受体）和CD36（簇决定簇36）在序列上显着同源，但NinaD尚未在功能细节上进行表征。因此，我们建立了果蝇S2细胞培养系统，用于生化表征ninaD基因产物。我们显示，由ninaD编码的两个剪接变异体同工型表现出不同的亚细胞定位。 NinaD-I（长蛋白变体）位于质膜上，而NinaD-II（短蛋白变体）位于细胞内膜上。在该细胞培养系统中，只有NinaD-1可以介导胶束对类胡萝卜素的细胞摄取。类胡萝卜素的摄取是浓度依赖性和饱和的。通过对不同突变体和转基因果蝇菌株的体内分析，我们提供了NinaD-I在吸收膳食类胡萝卜素以支持视觉发色团合成中的重要作用的证据。而且，我们的分析表明NinaD-1在生育酚代谢中的作用。即使果蝇是固醇营养缺陷型，我们也没有发现NinaD-1对这些化合物摄取的贡献的证据。总之，我们的研究建立了B类清除剂受体与脂溶性维生素在动物生理学中的多种功能之间的进化上保守的联系。

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