...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Induction of a protein-targeted catalytic response in autoimmune prone mice: Antibody-mediated cleavage of HIV-1 glycoprotein GP120
【24h】

Induction of a protein-targeted catalytic response in autoimmune prone mice: Antibody-mediated cleavage of HIV-1 glycoprotein GP120

机译：自身免疫易感小鼠中蛋白质靶向催化反应的诱导：HIV-1糖蛋白GP120的抗体介导裂解

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have induced a polyclonal IgG that degrades the HIV-1 surface antigen, glycoprotein gp120, by taking advantage of the susceptibility of SJL mice to a peptide-induced autoimmune disorder, experimental autoimmune encephalomyelitis (EAE). Specific pathogen-free SJL mice were immunized with structural fragments of gp120, fused in-frame with encephalitogenic peptide MBP85-101. It has resulted in a pronounced disease-associated immune response against antigens. A dramatic increase of gp120 degradation level by purified polyclonal IgG from immunized versus nonimmunized mice has been demonstrated by a newly developed fluorescence-based assay. This activity was inhibited by anti-mouse immunoglobulin antibodies as well as by Ser- and His-reactive covalent inhibitors. A dominant proteolysis site in recombinant gp120 incubated with purified polyclonal IgG from immunized mice was shown by SDS-PAGE. The SELDI-based mass spectrometry revealed that these antibodies exhibited significant specificity toward the Pro(484)-Leu(485) peptide bond. The sequence surrounding this site is present in nearly half of the HIV-1 variants. This novel strategy can be generalized for creating a catalytic vaccine against viral pathogens.
机译：我们已经利用SJL小鼠对肽诱导的自身免疫性疾病（实验性自身免疫性脑脊髓炎）的敏感性，诱导了降解HIV-1表面抗原糖蛋白gp120的多克隆IgG。用gp120的结构片段免疫特定的无病原体SJL小鼠，将其与致脑炎肽MBP85-101框内融合。它已导致针对抗原的明显的疾病相关免疫反应。通过新开发的基于荧光的测定法已证明，来自免疫小鼠和未免疫小鼠的纯化多克隆IgG显着提高了gp120降解水平。该活性被抗小鼠免疫球蛋白抗体以及Ser和His反应性共价抑制剂抑制。通过SDS-PAGE显示了与来自免疫小鼠的纯化的多克隆IgG孵育的重组gp120中的主要蛋白水解位点。基于SELDI的质谱分析表明，这些抗体对Pro（484）-Leu（485）肽键显示出显着的特异性。该位点周围的序列存在于近一半的HIV-1变体中。可以推广这种新颖的策略来创建针对病毒病原体的催化疫苗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号