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首页> 外文期刊>Biochemistry >Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins
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Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins

机译：探索新的发色团结构导致鉴定出改进的蓝色荧光蛋白

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摘要

The variant of Aequorea green fluorescent protein (GFP) known as blue fluorescent protein (BFP) was originally engineered by substituting histidine for tyrosine in the chromophore precursor sequence. Herein we report improved versions of BFP along with a variety of engineered fluorescent protein variants with novel and distinct chromophore structures that all share the property of a blue fluorescent hue. The two most intriguing of the new variants are a version of GFP in which the chromophore does not undergo excited-state proton transfer and a version of mCherry with a phenylalanine-derived chromophore. All of the new blue fluorescing proteins have been critically assessed for their utility in live cell fluorescent imaging. These new variants should greatly facilitate multicolor fluorescent imaging by legitimizing blue fluorescing proteins as practical and robust members of the fluorescent protein "toolkit".
机译：水母绿色荧光蛋白（GFP）的变体称为蓝色荧光蛋白（BFP），最初是通过在发色团前体序列中用组氨酸取代酪氨酸来进行工程设计的。本文中，我们报告了BFP的改进版本以及具有新颖独特的生色团结构的各种工程荧光蛋白变体，这些变体均具有蓝色荧光色调的特性。新变体中，两个最吸引人的地方是生色团不经历激发态质子转移的GFP版本和带有苯丙氨酸生色团的mCherry版本。所有新的蓝色荧光蛋白已在活细胞荧光成像中经过严格评估。这些新的变体应通过使蓝色荧光蛋白合法化为荧光蛋白“工具包”的实用且强大的成员，极大地促进多色荧光成像。

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