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首页> 外文期刊>Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases >Rapid identification of clinical Trichoderma longibrachiatum isolates by cellulose-acetate electrophoresis-mediated isoenzyme analysis.
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Rapid identification of clinical Trichoderma longibrachiatum isolates by cellulose-acetate electrophoresis-mediated isoenzyme analysis.

机译：通过纤维素乙酸酯电泳介导的同工酶分析快速鉴定临床长木霉菌分离株。

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摘要

Cellulose-acetate electrophoresis was used to investigate isoenzyme polymorphism among ten clinical and 11 non-clinical isolates of Trichoderma. Initial testing of 13 enzyme systems for activity and resolution of bands showed that seven were appropriate for identifying the different species. Each of the enzyme systems investigated (glucose-6-phosphate dehydrogenase, glucose-6-phosphate isomerase, 6-phosphogluconate dehydrogenase, peptidases A, B and D, and phosphoglucomutase) was diagnostic for at least one species. On the basis of the results of isoenzyme analysis, several isolates identified originally as Trichoderma pseudokoningii, T. koningii or T. citrinoviride were re-identified as T. longibrachiatum, in agreement with sequence analysis data for the internal transcribed spacer region of the isolates. The availability of a quick, inexpensive and reliable diagnostic tool for the identification of T. longibrachiatum isolates is important, as most clinical Trichoderma isolates belong to T. longibrachiatum. Furthermore, as many different enzyme systems are available, the method may also be suitable for the identification of other clinically relevant fungal species.
机译：醋酸纤维素电泳用于研究木霉属的10个临床分离株和11个非临床分离株中的同工酶多态性。初步测试了13种酶系统的活性和谱带分离度，发现有7种适合识别不同物种。所研究的每种酶系统（6-磷酸葡萄糖脱氢酶，6-磷酸葡萄糖异构酶，6-磷酸葡萄糖酸酯脱氢酶，肽酶A，B和D以及磷酸葡萄糖变位酶）均可诊断至少一种。根据同工酶分析的结果，与分离株内部转录间隔区的序列分析数据一致，将最初鉴定为假木霉，T。koningii或citrinoviride的几种分离株重新鉴定为长曲霉。快速，廉价和可靠的诊断工具可用于识别长支木霉菌菌株，这一点很重要，因为大多数临床木霉菌菌株均属于长支木霉菌。此外，由于有许多不同的酶系统可供使用，因此该方法也可能适用于鉴定其他临床相关的真菌物种。

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