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首页> 外文期刊>Biochemistry >Comparisons between Chemical Mapping and Binding to Isoenergetic Oligonucleotide Microarrays Reveal Unexpected Patterns of Binding to the Bacillus subtilis RNase P RNA Specificity Domain
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Comparisons between Chemical Mapping and Binding to Isoenergetic Oligonucleotide Microarrays Reveal Unexpected Patterns of Binding to the Bacillus subtilis RNase P RNA Specificity Domain

机译：化学作图和同能寡核苷酸芯片的结合之间的比较揭示了与枯草芽孢杆菌RNase P RNA特异性域结合的意外模式。

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摘要

Microarrays with isoenergetic pentamer and hexamer 2'-0-methyl oligonucleotide probes with LNA (locked nucleic acid) and 2,6-diaminopurine substitutions were used to probe the binding sites on the RNase P RNA specificity domain of Bacillus subtilis. Unexpected binding patterns were revealed. Because of their enhanced binding free energies, isoenergetic probes can break short duplexes, merge adjacent loops, and/or induce refolding. This suggests new approaches to the rational design of short oligonucleotide therapeutics but limits the utility of microarrays for providing constraints for RNA structure determination. The microarray results are compared to results from chemical mapping experiments, which do provide constraints. Results from both types of experiments indicate that the RNase P RNA folds similarly in 1 M Na~+ and 0 mM Mg~(2+).
机译：具有等能五聚体和具有LNA（锁定核酸）和2,6-二氨基嘌呤取代的六聚体2'-0-甲基寡核苷酸探针的微阵列用于探测枯草芽孢杆菌RNase P RNA特异性结构域上的结合位点。揭示了意外的绑定模式。由于其增强的结合自由能，等能探针可以破坏短双链体，合并相邻环和/或诱导重折叠。这提示了合理设计短寡核苷酸疗法的新方法，但限制了微阵列为RNA结构测定提供限制的实用性。将微阵列结果与化学制图实验的结果进行比较，后者确实提供了限制。两种实验的结果均表明，RNase P RNA在1 M Na〜+和0 mM Mg〜（2+）中折叠相似。

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