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首页> 外文期刊>Biochemistry >Characterization of Sulfolipids of Mycobacterium tuberculosis H37Rv by Multiple-Stage Linear Ion-Trap High-Resolution Mass Spectrometry with Electrospray Ionization Reveals That the Family of Sulfolipid II Predominates
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Characterization of Sulfolipids of Mycobacterium tuberculosis H37Rv by Multiple-Stage Linear Ion-Trap High-Resolution Mass Spectrometry with Electrospray Ionization Reveals That the Family of Sulfolipid II Predominates

机译：多级线性离子阱高分辨质谱联用电喷雾电离表征结核分枝杆菌H37Rv的硫脂揭示了硫脂II家族占主导地位。

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Mycobacterium tuberculosis, the causative agent of tuberculosis, is unique among bacterial pathogens in that it contains a wide array of complex lipids and lipoglycans on its cell wall. Among them, the sulfated glycolipid, termed the sulfolipid, is thought to mediate specific host—pathogen interactions during infection. Sulfolipids (SLs), including sulfolipid I (SL-I) and sulfolipid H (SL-II), are 2,3,6,6'-tetraacyltrehalose 2'-sulfates. SL-I was identified as a family of homologous 2-palmitoyl(stearoyl).3.phthioceranoyl.6,6'.bis. (hydroxyphthioceranoyl )trehalose 2'-sulfates and was believed to be the principal sulfolipid of M. tuberculosis strain H37Rv. We cultured and extracted sulfolipids using various conditions, including those originally described, and employed high-resolution multiple-stage linear ion-trap mass spectrometry with electrospray ionization to characterize the structure of the principal SL. We revealed that SL-II, a family of homologous 2-stearoyl(palmitoyl)-3,6,6'-tris(hydroxyphthioceranoyl)-trehalose 2'-sulfates, rather than SL-I is the principal sulfolipid class. We identified a great number of isomers resulting from permutation of the various hydroxyphthioceranoyl substituents at positions 6 and 6' of the trehalose backbone for each of the SL-II species in the entire family. We redefined the structure of this important lipid family that was misassigned using the traditional methods 40 years ago.
机译：结核分枝杆菌是结核的病原体，在细菌病原体中是独特的，因为它在其细胞壁上含有多种复杂的脂质和脂多糖。其中，被称为糖脂的硫酸化糖脂被认为在感染过程中介导了特定的宿主-病原体相互作用。硫脂（SLs），包括硫脂I（SL-1）和硫脂H（SL-II），是2,3,6,6'-四酰基海藻糖2'-硫酸盐。 SL-1被鉴定为同源的2-棕榈酰基（硬脂酰基）3 。phcerceranoyl.6，6'.bis的家族。 （羟基苯硫基神经酰基）海藻糖2'-硫酸盐，被认为是结核分枝杆菌菌株H37Rv的主要硫脂。我们使用各种条件（包括最初描述的条件）培养和提取了硫脂质，并采用了带有电喷雾电离的高分辨率多级线性离子阱质谱技术来表征主要SL的结构。我们揭示了SL-II，是同源的2-硬脂酰基（棕榈酰基）-3,6,6'-三（羟基苯硫基神经酰基）-海藻糖2'-硫酸盐的家族，而不是SL-1是主要的脂脂质类。我们确定了整个家族中每个SL-II物种在海藻糖主链6和6'位置上的各种羟基苯硫基ceranoyl取代基的置换产生的大量异构体。我们重新定义了这个重要的脂质家族的结构，该家族是40年前使用传统方法分配的。

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