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首页> 外文期刊>Biochemistry >Characterization of Arabidopsis Ca~(2+)/H~+ Exchanger CAX3
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Characterization of Arabidopsis Ca~(2+)/H~+ Exchanger CAX3

机译：拟南芥Ca〜（2 +）/ H〜+交换子CAX3的表征

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摘要

Plant calcium (Ca~(2+)) gradients, millimolar levels in the vacuole and micromolar levels in the cytoplasm, are regulated in part by high-capacity vacuolar cation/H+ exchangers (CAXs). Several CAX transporters, including CAX1, appear to contain an approximately 40-amino acid N-terminal regulatory region (NRR) that modulates transport through N-terminal autoinhibition. Deletion of the NRR from several CAXs (sCAX) enhances function in plant and yeast expression assays; however, to date, there are no functional assays for CAX3 (or sCAX3), which is 77% identical and 91% similar in sequence to CAX1. In this report, we create a series of truncations in the CAX3 NRR and demonstrate activation of CAX3 in both yeast and plants by truncating a large portion (up to 90 amino acids) of the NRR. Experiments with endomembrane-enriched vesicles isolated from yeast expressing activated CAX3 demonstrate that the gene encodes Ca~(2+)/H+ exchange with properties distinct from those of CAX1. The phenotypes produced by activated CAX3-expressing in transgenic tobacco lines are also distinct from those produced by sCAX1-expressing plants. These studies demonstrate shared and unique aspects of CAX1 and CAX3 transport and regulation.
机译：植物钙（Ca〜（2+））梯度，液泡中的毫摩尔水平和细胞质中的微摩尔水平部分受高容量液泡阳离子/ H +交换剂（CAXs）调节。几种CAX转运蛋白，包括CAX1，似乎包含约40个氨基酸的N末端调节区（NRR），可调节通过N末端自抑制作用的转运。从几种CAX（sCAX）中删除NRR可增强植物和酵母表达测定的功能;但是，到目前为止，还没有针对CAX3（或sCAX3）的功能分析，该分析与CAX1的序列同源性为77％，相似性为91％。在此报告中，我们在CAX3 NRR中创建了一系列截断，并通过截断NRR的大部分（最多90个氨基酸）证明了酵母和植物中CAX3的激活。从表达激活的CAX3的酵母中分离的富含膜的囊泡的实验表明，该基因编码Ca〜（2 +）/ H +交换，其特性不同于CAX1。在转基因烟草品系中由活化的CAX3表达产生的表型也与由sCAX1表达的植物产生的表型不同。这些研究证明了CAX1和CAX3的运输和调控具有共同而独特的方面。

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