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首页> 外文期刊>Biochemistry >Mg ~2(+)-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species
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Mg ~2(+)-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species

机译：在GTP或GTP类似物的存在下，Mts〜2（+）连接的FtsZ的自组装涉及寡聚物种窄尺寸分布的协同形成

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摘要

The assembly of the bacterial cell division FtsZ protein in the presence of constantly replenished GTP was studied as a function of Mg ~(2+) concentration (at neutral pH and 0.5 M potassium) under steady-state conditions by sedimentation velocity, concentration-gradient light scattering, fluorescence correlation spectroscopy, and dynamic light scattering. Sedimentation velocity measurements confirmed previous results indicating cooperative appearance of a narrow size distribution of finite oligomers with increasing protein concentration. The concentration dependence of light scattering and diffusion coefficients independently verified the cooperative appearance of a narrow distribution of high molecular weight oligomers, and in addition provided a measurement of the average size of these species, which corresponds to 100 ± 20 FtsZ protomers at millimolar Mg ~(2+) concentration. Parallel experiments on solutions containing guanosine-5′-[(α,β)- methyleno]triphosphate, sodium salt (GMPCPP), a slowly hydrolyzable analogue of GTP, in place of GTP, likewise indicated the concerted formation of a narrow size distribution of fibrillar oligomers with a larger average mass (corresponding to 160 ± 20 FtsZ monomers). The closely similar behavior of FtsZ in the presence of both GTP and GMPCPP suggests that the observations reflect equilibrium rather than nonequilibrium steady-state properties of both solutions and exhibit parallel manifestations of a common association scheme. (Graph Presented).
机译：在稳态条件下，通过沉降速度，浓度梯度研究了在不断补充GTP的情况下细菌细胞分裂FtsZ蛋白的组装与Mg〜（2+）浓度（在中性pH和0.5 M钾下）的函数的关系。光散射，荧光相关光谱和动态光散射。沉降速度测量结果证实了先前的结果，表明有限的低聚物的窄尺寸分布随蛋白质浓度的增加而协同出现。光散射和扩散系数的浓度依赖性独立地验证了高分子量低聚物的窄分布的协同外观，此外还提供了这些物质的平均尺寸的测量值，其对应于毫摩尔Mg〜100的20±20 FtsZ质子。 （2+）浓度。在含有鸟苷5'-[（（α，β）-甲基烯丙基）三磷酸钠盐（GMPCPP）（一种缓慢水解的GTP类似物）代替GTP的溶液中进行的并行实验同样表明，该化合物协同形成了窄尺寸的GTP。平均质量较大的原纤维低聚物（相当于160±20 FtsZ单体）。在同时存在GTP和GMPCPP的情况下，FtsZ的行为极为相似，这表明观察结果反映了两种溶液的平衡而不是非平衡稳态性质，并表现出共同缔合方案的平行表现。 （显示图形）。

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