...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Dss1 release activates DNA binding potential in Brh2
【24h】

Dss1 release activates DNA binding potential in Brh2

机译：Dss1释放激活Brh2中的DNA结合潜力

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dss1 is an intrinsically unstructured polypeptide that partners with the much larger Brh2 protein, the BRCA2 ortholog in Ustilago maydis, to form a tight complex. Mutants lacking Dss1 have essentially the same phenotype as mutants defective in Brh2, implying that through physical interaction Dss1 serves as a positive activator of Brh2. Dss1 associates with Brh2 through an interaction surface in the carboxy-terminal region. Certain derivatives of Brh2 lacking this interaction surface remain highly competent in DNA repair as long as a DNA-binding domain is present. However, the Dss1-independent activity raises the question of what function might be met in the native protein by having Brh2 under Dss1 control. Using a set of Brh2 fusions and truncated derivatives, we show here that Dss1 is capable of exerting control when there is a cognate Dss1-interacting surface present. We find that association of Dss1 attenuates the DNA binding potential of Brh2 and that the amino-terminal domain of Brh2 helps evict Dss1 from its carboxy-terminal interaction surface. The findings presented here add to the notion that Dss1 serves in a regulatory capacity to dictate order in association of Brh2's amino-terminal and carboxy-terminal domains with DNA.
机译：Dss1是一种固有的非结构化多肽，可与更大的Brh2蛋白（Ustilago maydis中的BRCA2 ortholog）结合形成紧密的复合物。缺少Dss1的突变体具有与Brh2缺陷型突变体基本相同的表型，这意味着通过物理相互作用Dss1可以作为Brh2的阳性激活剂。 Dss1通过羧基末端区域中的相互作用表面与Brh2缔合。只要存在DNA结合结构域，某些缺少此相互作用表面的Brh2衍生物仍具有很高的DNA修复能力。但是，不依赖于Dss1的活性提出了一个问题，即通过将Brh2置于Dss1的控制之下，天然蛋白可能具有什么功能。使用一组Brh2融合和截短的导数，我们在这里显示Dss1能够在存在相关的Dss1相互作用表面时发挥控制作用。我们发现，Dss1的关联减弱了Brh2的DNA结合潜力，并且Brh2的氨基末端结构域有助于从其羧基末端相互作用表面驱逐Dss1。此处提出的发现增加了Dss1以调节能力来指示Brh2的氨基末端和羧基末端结构域与DNA关联的顺序的概念。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号