...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >HIV-1 Vpu protein mediates the transport of potassium in Saccharomyces cerevisiae
【24h】

HIV-1 Vpu protein mediates the transport of potassium in Saccharomyces cerevisiae

机译：HIV-1 Vpu蛋白介导酿酒酵母中钾的转运

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human immunodeficiency virus type 1 (HIV-1) Vpu is an integral membrane protein that belongs to the viroporin family. Viroporins interact with cell membranes, triggering membrane permeabilization and promoting release of viral particles. In vitro electrophysiological methods have revealed changes in membrane ion currents when Vpu is present; however, in vivo the molecular mechanism of Vpu at the plasma membrane is still uncertain. We used the yeast Saccharomyces cerevisiae as a genetic model system to analyze how Vpu ion channel impacts cellular homeostasis. Inducible expression of Vpu impaired cell growth, suggesting that this viral protein is toxic to yeast cultures. This toxicity decreased with extracellular acidic pH. Also, Vpu toxicity diminished as the extracellular K~+ concentration was increased. However, expression of the Vpu protein suppresses the growth defect of K~+ uptake-deficient yeast (Δtrk1,2). The phenotype rescue of these highly hyperpolarized cells was almost total when they were grown in medium supplemented with high concentrations of KCl (100 mM) at pH 7.0 but was significantly reduced when the extracellular K~+ concentration or pH was decreased. These results indicate that Vpu has the ability to modify K ~+ transport in both yeast strains. Here, we show also that Vpu confers tolerance to the aminoglycoside antibiotic hygromycin B in Δtrk1,2 yeast. Our results suggest that Vpu interferes with cell growth of wild-type yeast but improves proliferation of the hyperpolarized trk1,2 mutant by inducing plasma membrane depolarization. Furthermore, evaluation of the ion channel activity of the Vpu protein in Δtrk1,2 yeast could aid in the development of a high-throughput screening assay for molecules that target the retroviral protein.
机译：1型人类免疫缺陷病毒（HIV-1）Vpu是一种整合膜蛋白，属于viroporin家族。 Viroporin与细胞膜相互作用，触发膜通透性并促进病毒颗粒的释放。体外电生理学方法揭示了当存在Vpu时膜离子电流的变化。然而，体内Vpu在质膜的分子机制仍不确定。我们使用了酿酒酵母作为遗传模型系统来分析Vpu离子通道如何影响细胞动态平衡。 Vpu的可诱导表达损害细胞生长，表明该病毒蛋白对酵母培养物有毒。这种毒性随着细胞外酸性pH的降低而降低。而且，随着细胞外K +浓度增加，Vpu毒性也降低。然而，Vpu蛋白的表达抑制了K +摄取不足的酵母（Δtrk1,2）的生长缺陷。当这些高度超极化的细胞在补充有高浓度KCl（100 mM）的pH 7.0的培养基中生长时，它们的表型拯救几乎是完全的，但是当细胞外K +浓度或pH降低时，它们的表型拯救却显着降低。这些结果表明，Vpu具有在两种酵母菌株中修饰K +转运的能力。在这里，我们还显示Vpu赋予Δtrk1,2酵母中的氨基糖苷类抗生素潮霉素B耐受性。我们的结果表明，Vpu会干扰野生型酵母的细胞生长，但通过诱导质膜去极化来改善超极化的trk1,2突变体的增殖。此外，对Δtrk1,2酵母中Vpu蛋白的离子通道活性的评估可以帮助开发针对逆转录病毒蛋白的分子的高通量筛选测定方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号