Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

Thomas R. Sundermeier; Hui Jin; Matthew L. Kleinjan; Debarshi Mustafi; Donny D. Licatalosi; Krzysztof Palczewski

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Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

机译：通过交联免疫沉淀分离的RNA的Argonaute高通量测序揭示了哺乳动物视网膜中miRNA基因调控的快照。

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Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute highthroughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 highconfidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases.

机译：越来越多的证据表明，miRNA基因调控在促进哺乳动物视网膜的发育，功能和细胞存活中的作用。但是，对于哪些视网膜基因是miRNA的靶标知之甚少。在这里，我们采用了系统的，无偏见的生化方法来鉴定牛视网膜中miRNA基因调控的目标，牛视网膜是视觉研究的常见模型物种。使用通过交联免疫沉淀分析分离的RNA的Argonaute高通量测序，我们鉴定了261个基因中的348个高信度miRNA目标位点。该列表丰富了杆和锥光感受器基因，包括28个视网膜疾病基因，进一步证明了miRNA在致盲疾病病理中的作用。

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《Biochemistry》 |2014年第37期|共3页
作者
Thomas R. Sundermeier; Hui Jin; Matthew L. Kleinjan; Debarshi Mustafi; Donny D. Licatalosi; Krzysztof Palczewski;
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High-Throughput; evidence; diseases;

机译：高通量;证据;疾病;

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1. Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina [J] . Thomas R. Sundermeier, Hui Jin, Matthew L. Kleinjan, Biochemistry . 2014,第37期

机译：通过交联免疫沉淀分离的RNA的Argonaute高通量测序揭示了哺乳动物视网膜中miRNA基因调控的快照。
2. Identification of HIV-1 sequences targeted by the miRNA Induced silencing complex (miRISC) using Argonaute 2 cross-linking and immunoprecipitation (HITS-CLIP) [J] . Agathe Eckenfelder, Natalia Pinzon Restrepo, Damien Ulveling, Retrovirology . 2013,第S1期

机译：使用Argonaute 2交联和免疫沉淀（Hits-Clip），鉴定MiRNA诱导的沉默复合物（MiRisc）靶向的HIV-1序列（MiRISC）
3. Deep-sequencing of human Argonaute-associated small RNAs provides insight into miRNA sorting and reveals Argonaute association with RNA fragments of diverse origin. [J] . Burroughs AM, Ando Y, de Hoon MJ, RNA biology . 2011,第1期

机译：人类与Argonaute相关的小RNA的深度测序提供了对miRNA排序的深入了解，并揭示了Argonaute与各种来源的RNA片段的关联。
4. Coordinated regulation of mirnas to induce chondrogenesis and inhibit angiogenesis in stem cells and ECM materials under hypoxic environment [C] . Manman Gao, Shaoming Chen, Zhiyu Zhou, World biomaterials congress . 2016

机译：低氧环境下协同调控神经元诱导干细胞和ECM材料中的软骨形成并抑制血管生成
5. Novel roles of the conserved Argonaute proteins during mammalian development: From miRNA biogenesis to gene silencing. [D] . Cheloufi, Sihem. 2010

机译：保守的Argonaute蛋白在哺乳动物发育过程中的新作用：从miRNA生物发生到基因沉默。
6. Argonaute High-Throughput Sequencing of RNAs Isolatedby Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA GeneRegulation in the Mammalian Retina [O] . ThomasR. Sundermeier, Hui Jin, Matthew L. Kleinjan, -1

机译：分离的RNA的Argonaute高通量测序通过交联免疫沉淀揭示了miRNA基因的快照哺乳动物视网膜的调节
7. High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation (HITS-CLIP) reveals Argonaute-associated microRNAs and targets in Schistosoma japonicum [O] . Jing Zhao, Rong Luo, Xindong Xu, 2015

机译：通过交联免疫沉淀（HITS-CLIP）分离的RNA的高通量测序揭示了日本血吸虫中与Argonaute相关的microRNA和靶标

Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

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