Polyhydroxybutyrate (PHB) synthases (PhaCs) catalyze the conversion of 3-(R)-hydroxybutyryl CoA (HBCoA) to PHB, which is deposited as granules in the cytoplasm of microorganisms. The class I PhaC from Caulobacter crescentus (PhaC(Cc)) is a highly soluble protein with a turnover number of 75 s(-1) and no lag phase in coenzyme A (CoA) release. Studies with [1-14C]HBCoA and PhaC(Cc) monitored by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography reveal that the rate of elongation is much faster than the rate of initiation. Priming with the artificial primer [H-3](s)TCoA and monitoring for CoA release reveal a single CoA/PhaC, suggesting that the protein is uniformly loaded and that the elongation process could be studied. Reaction of sT-PhaC(Cc) with [1-C-14]HBCoA revealed that priming with sTCoA increased the uniformity of elongation, allowing distinct polymerization species to be observed by SDSPAGE and autoradiography. However, in the absence of HBCoA, [H-3](s)T-PhaC unexpectedly generates [H-3](s)DCoA with a rate constant of 0.017 s(-1). We propose that the [H-3](s)DCoA forms via attack of CoA on the oxoester of the [H-3](s)T-PhaC chain, leaving the synthase attached to a single HB unit. Comparison of the relative rate constants of thiolysis by CoA and elongation by PhaC(Cc), and the size of the PHB polymer generated in vivo, suggests a mechanism for chain termination and reinitiation.
展开▼
