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首页> 外文期刊>Biomaterials >Elastin-derived protein coating onto poly(ethylene terephthalate). Technical, microstructural and biological studies.
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Elastin-derived protein coating onto poly(ethylene terephthalate). Technical, microstructural and biological studies.

机译：弹性蛋白衍生的蛋白涂层在聚对苯二甲酸乙二醇酯上。技术，微观结构和生物学研究。

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Recently, it has been shown in our laboratory that certain proteins solubilized from elastin (ESP) formed a tight association with certain polymers such as elastin or polyethylene glycol terephthalate (PET) ... . The present paper deals with the description of the optimal chemical conditions of this unexpected association, its microstructure and its biological properties. A microstructural study of the composite ESP-PET material was performed using scanning and transmission electron microscopy. The thickness of the yield composite was evaluated (0.4-2 microm) but its imperviousness was unsatisfactory using ESP alone. So, we tentatively coated PET with the elastin-ESP complex. The microscopic views confirmed that the polymer was better filled by the organic matrix, the thickness of the layer being markedly improved (3 microm). Simultaneously, we attempted to verify whether the yielded composite retains the biological properties previously demonstrated with the 'Biopatches' and probably due to ESP. Thus, the culture of endothelial cells on an ESP-coating (with elastin or not) showed that a 100 microg/cm2 ESP concentration was able to promote endothelial cell growth in perfect conditions, maintaining their phenotypic character. While several physico-chemical determinations are in progress in our laboratory to identify and characterize the protein involved, a prototype of small-calibre vascular prosthesis was elaborated with elastin-ESP-PET composite and will be placed in a dog at the abdominal femoral junction to evaluate the in vivo performance of such an attractive material in artery restoration.
机译：最近，在我们的实验室中已显示，从弹性蛋白（ESP）溶解的某些蛋白质与某些聚合物（例如弹性蛋白或聚对苯二甲酸乙二醇酯（PET））形成紧密的结合。本文介绍了这种意外结合的最佳化学条件，其微观结构和生物学特性。使用扫描和透射电子显微镜对复合ESP-PET材料进行了微观结构研究。评价了屈服复合材料的厚度（0.4-2微米），但仅使用ESP不能令人满意。因此，我们尝试用弹性蛋白-ESP复合物涂覆PET。显微图证实，聚合物被有机基质更好地填充，该层的厚度显着改善（3微米）。同时，我们试图验证所得复合材料是否保留了以前用“生物补丁”证明的生物学特性，并且可能归因于ESP。因此，在ESP涂层（有或没有弹性蛋白）上培养内皮细胞表明，在理想条件下，浓度为100 microg / cm2的ESP能够促进内皮细胞生长，并保持其表型特征。尽管我们实验室中正在进行一些物理化学测定，以鉴定和表征所涉及的蛋白质，但小口径血管假体的原型是用弹性蛋白-ESP-PET复合材料制成的，将被放置在狗腹股骨交界处。评估这种有吸引力的材料在动脉修复中的体内性能。

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