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首页> 外文期刊>Biomaterials >The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation.
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The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation.

机译：电纺底物纤维直径对神经干细胞分化和增殖的影响。

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Neural stem/progenitor cells (NSCs) are capable of self-renewal and differentiation into all types of neural lineage under different biochemical and topographical cues. In this study, we cultured rat hippocampus-derived adult NSCs (rNSCs) on laminin-coated electrospun Polyethersulfone (PES) fiber meshes with average fiber diameters of 283+/-45 nm, 749+/-153 nm and 1452+/-312 nm; and demonstrated that fiber diameter of PES mesh significantly influences rNSC differentiation and proliferation. Under the differentiation condition (in the presence of 1 microM retinoic acid and 1% fetal bovine serum), rNSCs showed a 40% increase in oligodendrocyte differentiation on 283-nm fibers and 20% increase in neuronal differentiation on 749-nm fibers, in comparison to tissue culture polystyrene surface. SEM imaging revealed that cells stretched multi-directionally to follow underlying 283-nm fibers, but extended along a single fiber axis on larger fibers. When cultured on fiber meshes in serum free medium in the presence of 20 ng/mL of FGF-2, rNSCs showed lower proliferation and more rounded morphology compared to that cultured on laminin-coated 2D surface. As the fiber diameter decreased, higher degree of proliferation and cell spreading and lower degree of cell aggregation were observed. This collective evidence indicates fiber topography can play a vital role in regulating differentiation and proliferation of rNSCs in culture.
机译：神经干/祖细胞（NSC）能够自我更新并在不同的生化和地形线索下分化为所有类型的神经谱系。在这项研究中，我们在层粘连蛋白包被的电纺聚醚砜（PES）纤维网上培养了大鼠海马来源的成年NSC（rNSC），其平均纤维直径为283 +/- 45 nm，749 +/- 153 nm和1452 +/- 312纳米并证明PES网眼的纤维直径显着影响rNSC的分化和增殖。相比之下，在分化条件下（存在1 microM视黄酸和1％胎牛血清），rNSCs在283 nm纤维上的少突胶质细胞分化增加40％，在749 nm纤维上的神经元分化增加20％。组织培养聚苯乙烯表面。 SEM成像显示，细胞可以多方向拉伸以跟随下面的283 nm光纤，但在较大的光纤上沿单个光纤轴延伸。当在存在20 ng / mL FGF-2的无血清培养基中的纤维网上进行培养时，与在层粘连蛋白涂层的2D表面上培养的rNSC相比，rNSCs的增殖率更低，形态更圆润。随着纤维直径的减小，观察到较高的增殖和细胞扩散程度以及较低的细胞聚集程度。这种集体证据表明，纤维形貌在调节培养物中rNSC的分化和增殖中起着至关重要的作用。

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