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首页> 外文期刊>Biomaterials >Endocytic carboxylated nanodiamond for the labeling and tracking of cell division and differentiation in cancer and stem cells.
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Endocytic carboxylated nanodiamond for the labeling and tracking of cell division and differentiation in cancer and stem cells.

机译：内吞羧化纳米金刚石，用于标记和跟踪癌细胞和干细胞中的细胞分裂和分化。

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摘要

Nanodiamond (ND) is carbon nanomaterial developing for biological applications in recent years. In this study, we investigated the location and distribution of 100 nm carboxylated ND particles in cell division and differentiation. ND particles were taken into cells by macropinocytosis and clathrin-mediated endocytosis pathways. However, the cell growth ability was not altered by endocytic ND particles after long-term cell culture for 10 days in both A549 lung cancer cells and 3T3-L1 embryonic fibroblasts. ND particles were equal separating into two daughter cells of cell division approximately. Finally, the cell retained a single ND's cluster in cytoplasm after sub-cultured for several generations. Interestingly, ND's clusters were carried inside of cell but without inducing damages after long-term cell culture. Moreover, ND particles did not interfere with the gene or protein expressions on the regulation of cell cycle progression and adipogenic differentiation. Together, these findings provide that endocytic ND particles are non-cytotoxic in cell division and differentiation, which can be applied for the labeling and tracking of cancer and stem cells.
机译：纳米金刚石（ND）是近年来用于生物应用的碳纳米材料。在这项研究中，我们调查了100 nm羧化ND颗粒在细胞分裂和分化中的位置和分布。通过巨胞饮作用和网格蛋白介导的内吞途径将ND颗粒带入细胞。但是，在A549肺癌细胞和3T3-L1胚胎成纤维细胞中，长期细胞培养10天后，内吞ND颗粒不会改变细胞的生长能力。 ND颗粒大约分成两个子单元，分别相等。最后，该细胞继代培养数代后，在细胞质中保留了单个ND簇。有趣的是，ND的簇被携带在细胞内部，但是在长期的细胞培养后却没有引起损伤。而且，ND颗粒在细胞周期进程和成脂分化的调节中不干扰基因或蛋白质表达。总之，这些发现提供了内吞性ND颗粒在细胞分裂和分化方面无细胞毒性，可用于癌症和干细胞的标记和追踪。

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