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首页> 外文期刊>Biomaterials >Long-term calibration considerations during subcutaneous microdialysis sampling in mobile rats.
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Long-term calibration considerations during subcutaneous microdialysis sampling in mobile rats.

机译：流动大鼠皮下微透析采样期间的长期校准注意事项。

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摘要

The level at which implanted sensors and sampling devices maintain their calibration is an important research area. In this work, microdialysis probes with identical geometry and different membranes, polycarbonate/polyether (PC) or polyethersulfone (PES), were used with internal standards (Vitamin B(12) (MW 1355), antipyrine (MW 188) and 2-deoxyglucose (2-DG, MW 164)) and endogenous glucose to investigate changes in their long-term calibration after implantation into the subcutaneous space of Sprague-Dawley rats. Histological analysis confirmed an inflammatory response to the microdialysis probes and the presence of a collagen capsule. The membrane extraction efficiency (percentage delivered to the tissue space) for antipyrine and 2-DG was not altered throughout the implant lifetime for either PC- or PES membranes. Yet, Vitamin B(12) extraction efficiency and collected glucose concentrations decreased during the implant lifetime. Antipyrine was administered i.v. and its concentrations obtained in both PC- and PES-membrane probes were significantly reduced between the implant day and seven (PC) or 10 (PES) days post-implantation suggesting that solute supply is critical for in vivo extraction efficiency. For the low molecular weight solutes such as antipyrine and glucose, localized delivery is not affected by the foreign body reaction, but recovery is significantly reduced. For Vitamin B(12), a larger solute, the fibrotic capsule formed around the probe significantly restricts diffusion from the implanted microdialysis probes.
机译：植入的传感器和采样设备保持其校准的水平是重要的研究领域。在这项工作中，将具有相同几何形状和不同膜，聚碳酸酯/聚醚（PC）或聚醚砜（PES）的微透析探针与内标（维生素B（12）（MW 1355），安替比林（MW 188）和2-脱氧葡萄糖）一起使用（2-DG，MW 164））和内源性葡萄糖以研究植入Sprague-Dawley大鼠皮下空间后其长期校准的变化。组织学分析证实了对微透析探针的炎症反应和胶原蛋白胶囊的存在。安替比林和2-DG的膜提取效率（输送至组织间隙的百分比）在PC-或PES膜的整个植入过程中均未改变。然而，维生素B（12）的提取效率和所收集的葡萄糖浓度在植入物寿命期间下降。安替比林经静脉给药。在植入后第7天（PC）或植入后第10天（PES），在PC和PES膜探针中获得的浓度均显着降低，这表明溶质的供应对于体内提取效率至关重要。对于安替比林和葡萄糖等低分子量溶质，局部递送不受异物反应的影响，但回收率显着降低。对于较大的溶质维生素B（12），探针周围形成的纤维化囊明显限制了植入的微透析探针的扩散。

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