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首页> 外文期刊>Biomaterials >The effect of cholesterol on protein-coated gold nanoparticle binding to liquid crystal-supported models of cell membranes.
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The effect of cholesterol on protein-coated gold nanoparticle binding to liquid crystal-supported models of cell membranes.

机译：胆固醇对蛋白涂层的金纳米粒子与液晶支持的细胞膜模型结合的影响。

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摘要

We report an easily visualized liquid crystal (LC)-based system to study the biophysical interactions between protein-coated gold nanoparticles (AuNPs) and LC-supported cell membrane model. The model consists of mixed phospholipid/cholesterol monolayer self-assembled at aqueous-LC interface. Protein-coated AuNPs were found to disrupt the mixed phospholipid/cholesterol monolayer. As a result, orientational transitions of LCs were triggered and optical responses of LCs from dark to bright were observed. The mixed monolayers with higher cholesterol contents were found to be more susceptible to the disruption by protein-coated AuNPs, and hydrophobic interaction played a major role in the monolayer disruption. We also found that the time for non-specific binding of fibrinogen-coated AuNPs to the mixed phospholipid/cholesterol monolayer was similar to that of specific binding of neutravidin-coated AuNPs to the mixed phospholipid/biotin-capped phospholipid monolayer. Results obtained from this study may offer new understanding in the potential nanotoxicity pathway, where the biophysical interaction between nanomaterials and cell membrane is an important step.
机译：我们报告一个易于可视化的液晶（LC）为基础的系统，以研究蛋白质涂层金纳米粒子（AuNPs）和LC支持的细胞膜模型之间的生物物理相互作用。该模型由在水-LC界面自组装的混合磷脂/胆固醇单层组成。发现蛋白包被的AuNPs破坏了混合的磷脂/胆固醇单层。结果，触发了LC的取向转变，并且观察到了LC从暗到亮的光学响应。发现具有较高胆固醇含量的混合单层更易于被蛋白包被的AuNP破坏，并且疏水相互作用在单层破坏中起主要作用。我们还发现，纤维蛋白原包被的AuNPs与磷脂/胆固醇混合单层非特异性结合的时间与中性生物素包被的AuNPs与磷脂/生物素封端的混合磷脂单层特异性结合的时间相似。这项研究获得的结果可能会为潜在的纳米毒性途径提供新的认识，而纳米材料和细胞膜之间的生物物理相互作用是重要的一步。

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