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首页> 外文期刊>Biomaterials >Early circulating biomarker detection using a wearable microprojection array skin patch
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Early circulating biomarker detection using a wearable microprojection array skin patch

机译：使用可穿戴式微突物阵列皮肤贴片进行早期循环生物标记检测

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Microprojection array (MPA) skin patches selectively capture circulating biomarkers from the dermal layers of the skin, avoiding the need to extract, handle or process blood. Here we investigate the effect of improving biomarker capture invivo on MPA detection of a model biomarker (antigen-specific-IgG raised in response to Fluvax vaccine) in a murine model. First, we investigate targeting MPA penetration to biomarker rich regions of the skin by varying MPA penetration depth. We observed a 4-fold increase in biomarker capture from predominantly epidermal to deep dermal penetration (27±9μm-153±30μm penetration range). We then study the kinetics of biomarker capture by varying the contact time with skin from rapid application (less than 20min) to long term application (up to 24h) with a wearable MPA patch. We observed MPAs reproducibly captured detectable amounts of our model biomarker after 10minapplication and a greater than 6-fold increase in capture was observed up to 6happlication. Combining the effect of penetration depth and application time we obtained comparable early detection (after vaccination) of our model biomarker as a standard enzyme-linked immunosorbent assay (ELISA). We expect that integration of these devices with existing detection technologies has potential advantages in rapid diagnostic tests, particularly in cases where laboratory-based sample collection and processing is not available.
机译：微突出物阵列（MPA）皮肤贴剂可选择性地从皮肤的真皮层捕获循环中的生物标志物，而无需提取，处理或加工血液。在这里，我们研究了在鼠模型中改进生物标志物捕获体内对MPA检测模型生物标志物（响应Fluvax疫苗而产生的抗原特异性IgG）的影响。首先，我们研究通过改变MPA渗透深度将MPA渗透靶向皮肤生物标记丰富的区域。我们观察到，从主要是表皮渗透到深层真皮渗透（27±9μm-153±30μm渗透范围），生物标志物捕获量增加了4倍。然后，我们通过使用可穿戴式MPA贴片，从快速应用（少于20分钟）到长期应用（长达24小时）改变与皮肤的接触时间，研究生物标志物捕获的动力学。我们观察到MPA可重复捕获10分钟，可检测到我们模型生物标志物的可检测量，并且观察到长达6 h的捕获量增加了6倍以上。结合渗透深度和施用时间的影响，我们获得了可比较的早期检测（疫苗接种后）的模型生物标记物，作为标准的酶联免疫吸附测定（ELISA）。我们希望将这些设备与现有检测技术集成在快速诊断测试中具有潜在的优势，尤其是在无法进行基于实验室的样品采集和处理的情况下。

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