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首页> 外文期刊>Biomaterials >Comparative efficacy of blood cell immunocamouflage by membrane grafting of methoxypoly(ethylene glycol) and polyethyloxazoline
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Comparative efficacy of blood cell immunocamouflage by membrane grafting of methoxypoly(ethylene glycol) and polyethyloxazoline

机译：通过膜接枝甲氧基聚乙二醇和聚乙基恶唑啉进行血细胞免疫伪装的比较功效

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摘要

The grafting of low-immunogenic polymers to cells dramatically reduces antigenic recognition and immunogenicity of allogeneic donor cells consequent to steric and charge camouflage (i.e., immunocamouflage). While methoxypoly(ethylene glycol) [mPEG] has historically been utilized for the immunocamouflage of cells, other low-immunogenic polymers such as polyethyloxazoline propionic acid (PEOZ) may also be capable of conferring immunoprotection. Moreover, PEOZ may have attributes that could have enhanced pharmacological and biological utility relative to mPEG. To evaluate the immunocamouflage efficacy of PEOZ relative to mPEG, human red blood cells (RBC) and leukocytes were modified with mPEG or PEOZ. The differential effects of mPEG and PEOZ was assessed via grafting efficacy, cell morphology and viability, immunocamouflage of surface antigens, and the prevention of invitro immune recognition (RhD and HLA). Although membrane grafting of mPEG and PEOZ were similar, mPEG demonstrated superior immunocamouflage efficacy as measured by antibody binding and phagocytosis of opsonized RBC while PEOZ showed improved RBC morphology. While mPEG appears to be superior to PEOZ in the immunocamouflage of cells, PEOZ may still be a valuable addition to our repertoire of immunomodulatory polymers. Moreover, our results demonstrate the importance of indirect immunocamouflage of antigens found in membrane protein complexes.
机译：将低免疫原性聚合物移植到细胞上会极大地降低同种异体供体细胞的抗原识别和免疫原性，这是由于其空间和电荷伪装（即免疫伪装）所致。尽管甲氧基聚（乙二醇）[mPEG]历史上已用于细胞的免疫伪装，但其他低免疫原性聚合物（例如聚乙基恶唑啉丙酸（PEOZ））也可能具有免疫保护作用。此外，PEOZ可能具有相对于mPEG具有增强的药理和生物学效用的属性。为了评估PEOZ相对于mPEG的免疫伪装功效，使用mPEG或PEOZ修饰了人类红细胞（RBC）和白细胞。通过移植功效，细胞形态和生存力，表面抗原的免疫伪装以及预防体外免疫识别（RhD和HLA）来评估mPEG和PEOZ的差异作用。尽管mPEG和PEOZ的膜移植相似，但通过抗体结合和调理过的RBC的吞噬作用，mPEG表现出优异的免疫伪装功效，而PEOZ显示出改善的RBC形态。尽管在细胞的免疫伪装方面mPEG似乎优于PEOZ，但PEOZ可能仍然是我们免疫调节聚合物库中的宝贵补充。此外，我们的结果证明了在膜蛋白复合物中发现的抗原的间接免疫伪装的重要性。

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