• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases >Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones
【24h】

Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones

机译：南非Tygerberg医院的金黄色葡萄球菌的种群结构分析显示，人群多样，Panton-Valentine leukocidin基因的患病率很高，并且独特的耐甲氧西林金黄色葡萄球菌克隆

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Studies reporting on the population structure of Staphylococcus aureus in South Africa have focused only on methicillin-resistant S. aureus (MRSA). This study describes the population structure of S. aureus, including methicillin-susceptible S. aureus (MSSA) isolated from patients at Tygerberg Academic Hospital, Western Cape province. Pulsed-field gel electrophoresis (PFGE), detection of Panton-Valentine leukocidin (PVL), spa typing, multilocus sequence typing (MLST), agr typing and SCCmec typing were used to characterize strains. Of 367 non-repetitive S. aureus isolates collected over a period of 1 year, 56 (15.3%) were MRSA. Skin and soft tissue infections were the most frequent source (54.8%), followed by bone and joint (15.3%) and respiratory tract infections (7.7%). For strain typing, PFGE was the most discriminative method, and resulted in 31 pulsotypes (n = 345, 94.0%), as compared with 16 spa clonal complexes (CCs) (n = 344, 93.4%). Four MLST CCs were identified after eBURST of sequence types (STs) of selected isolates. One hundred and sixty isolates (MSSA, n = 155, 42.2%) were PVL-positive, and agr types I-IV and SCCmec types I-V were identified. Our S. aureus population consisted of genotypically diverse strains, with PVL being a common characteristic of MSSA. MSSA and MRSA isolates clustered in different clones. However, the dominant MRSA clone (ST612) also contained an MSSA isolate, and had a unique genotype. Common global epidemic MRSA clones, such as ST239-MRSA-III and ST36-MRSA-II, were identified. A local clone, ST612-MRSA-IV, was found to be the dominant MRSA clone. Clinical Microbiology and Infection
机译：关于南非金黄色葡萄球菌种群结构的研究报告仅关注耐甲氧西林的金黄色葡萄球菌（MRSA）。这项研究描述了金黄色葡萄球菌的种群结构，包括从西开普省泰格堡学术医院的患者中分离出的对甲氧西林敏感的金黄色葡萄球菌（MSSA）。脉冲场凝胶电泳（PFGE），Panton-Valentine leukocidin的检测（PVL），spa分型，多位点序列分型（MLST），agr分型和SCCmec分型被用来表征菌株。在1年内收集的367份非重复性金黄色葡萄球菌分离株中，有56份（15.3％）是MRSA。皮肤和软组织感染是最常见的来源（54.8％），其次是骨骼和关节（15.3％）和呼吸道感染（7.7％）。对于菌株分型，PFGE是最有区别的方法，与16种温泉克隆复合物（CC）（n = 344，93.4％）相比，可产生31种搏动型（n = 345，94.0％）。在对选定的分离物的序列类型（ST）进行eBURST之后，鉴定了四个MLST CC。一百六十个菌株（MSSA，n = 155，42.2％）是PVL阳性的，并且鉴定了agr I-IV型和SCCmec I-V型。我们的金黄色葡萄球菌种群由不同基因型的菌株组成，PVL是MSSA的共同特征。 MSSA和MRSA隔离株聚集在不同的克隆中。但是，显性MRSA克隆（ST612）也包含MSSA分离株，并具有独特的基因型。确定了常见的全球流行性MRSA克隆，例如ST239-MRSA-III和ST36-MRSA-II。发现本地克隆ST612-MRSA-IV是主要的MRSA克隆。临床微生物学和感染

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号