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首页> 外文期刊>Bioorganic and Medicinal Chemistry Letters >Facile design of organic-inorganic hybrid gels for molecular recognition of nucleoside triphosphates
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Facile design of organic-inorganic hybrid gels for molecular recognition of nucleoside triphosphates

机译：用于分子识别三磷酸核苷的有机-无机杂化凝胶的简便设计

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摘要

We report the molecular recognition for nucleoside triphosphates inside the ligand-modified water-soluble organic-inorganic hybrid gels composed of polyhedral oligomeric silsesquioxane (POSS). The series of ligands were designed to make hydrogen bonds with each nucleobase and introduced into the hybrid gels. From the titration experiments, the binding constants between the ligand inside the gels and nucleosides were evaluated. Accordingly, it was found that the ligands inside the gels can form a stable complex only with the target nucleoside triphosphate which has the complementary pattern of the hydrogen bonds (K-a = G-gel/cytidine triphosphate (CTP), 3.5 x 10(8) M-1; U-gel/adenosine triphosphate (ATP), 1.6 x 10(3) M-1; A-gel/uridine triphosphate (UTP), 1.9 x 10(7), respectively). With other nucleoside derivatives which have different numbers of phosphate units or different types of nucleobases, the much weaker interaction was detected. These data indicate that the complex formation only with nucleoside triphosphates should occur inside the hybrid gels, and selective recognition of each nucleoside triphosphate can be accomplished. (C) 2015 Elsevier Ltd. All rights reserved.
机译：我们报告了由多面体低聚倍半硅氧烷（POSS）组成的配体修饰的水溶性有机-无机杂化凝胶中的核苷三磷酸分子识别。设计一系列配体以与每个核碱基形成氢键，并将其引入杂化凝胶中。从滴定实验中，评估了凝胶内配体与核苷之间的结合常数。因此，发现凝胶内的配体仅能与具有氢键互补图案的目标三磷酸核苷形成稳定的络合物（Ka = G-凝胶/胞苷三磷酸（CTP），3.5 x 10（8） M-1; U-凝胶/三磷酸腺苷（ATP），1.6 x 10（3）M-1; A-凝胶/三磷酸尿苷（UTP），分别为1.9 x 10（7））。对于具有不同数量的磷酸酯单元或不同类型的核碱基的其他核苷衍生物，检测到的相互作用弱得多。这些数据表明，仅与三磷酸核苷的复合物形成应该发生在杂化凝胶内部，并且可以实现每个三磷酸核苷的选择性识别。 （C）2015 Elsevier Ltd.保留所有权利。

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